DNA Replication

Question 1

what is the goal of DNA replication?

Answer 1

semiconservative; create exact copy using a template (cheat sheet)

Question 2

who are the “workers” for DNA replication?

Answer 2

DNA helicase, DNA ligase, RNA primase, DNA POL I, DNA POL III

Question 3

1. Fast/hard worker (500-1000 nucleotides/second
2. poor starter/great follower: needs RNA primers
3. occasionally makes mistakes
4. identify and fix mistakes (proof reading exonuclease function)

Answer 3

DNA POL III (younger)

Question 4

1. slow & “lags” behind POL III, but makes fewer mistakes
2. poor follower/ great starter

Answer 4

DNA POL I (older)

Question 5

removes RNA primers & replaces with DNA nucleotides

Answer 5

POL. I

Question 6

1. needs clear direction (template)
2. focused (works 5’ to 3’; adds nucleotides to 3’ end only)

Answer 6

DNA POL. I & III

Question 7

synthesized in pieces; discontinuous

Answer 7

lagging strand

Question 8

continuously synthesized

Answer 8

leading strand

Question 9

DNA helicase breaks hydrogen bonds at the replication origin to form replication forks

Answer 9

step I DNA replication

Question 10

complementary RNA primers bind to DNA (RNA primase)

Answer 10

Step II DNA replication

Question 11

DNA POL III adds complementary DNA nucleotides to 3’ end of RNA primer

Answer 11

Step III DNA replication

Question 12

DNA POL I removes RNA primers & replaces with complementary DNA nucleotides

Answer 12

Step IV DNA replication

Question 13

DNA ligase binds all DNA pieces to create 2 continuous new strands

Answer 13

Step V DNA replication