DNA replication I Flashcards

(9 cards)

1
Q

semi conservative method

A

double stranded DNA is unwound to produce 2 single strands and each parent strand is used to produce a daughter strand

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2
Q

what is the only way a DNA strand can be extended

A

by adding nucleotides to the 3’ OH end

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3
Q

” dna is semi discontinuous” explanation

A

leading strand- made continuously, primer extended by DNA polymerase from left to right
lagging strand

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4
Q

what is dna replication primed by

A

RNA

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5
Q

does RNA synthesis need a primer

A

no

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6
Q

okazaki fragment

A

short, newly synthesized DNA sequences that are created on the lagging strand during DNA replication.

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7
Q

what closes the nicks in the DNA backbone between okazaki fragments

A

DNA ligase

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8
Q

replication fork

A

the point at which the double streamed DNA is unwound by the helicase enzyme

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9
Q

is DNA replication bidirectional + explanation

A

YES
leading strand synthesised continuously, lagging strand synthesised discontinuously in a series of okazaki fragments

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