DNA Structure and Replication

Question 1

What base pairing is usually found at origin of replication sites? Why?

Answer 1

A-T. Because it only has two Hydrogen bonds rather than 3.

Question 2

What is the backbone of DNA?

Answer 2

Sugar-phosphate.

Question 3

In which direction does DNA Polymerase work?

Answer 3

3’ to 5’

Question 4

In which direction does DNA Polymerase build?

Answer 4

5’ to 3’

Question 5

What are two mechanisms that fix mistakes generated during DNA replication?

Answer 5

DNA Polymerase and DNA Repair Enzymes.

Question 6

What must be put into the PCR mixture for it to work?

Answer 6

Mix DNA template with primers, DNA building blocks, and DNA Polymerase.

Question 7

What are the 3 stages of PCR?

Answer 7

1. Heat at 95C for 30 sec (denaturation).
2. Cool to 60C for 30 sec (annealing).
3. Heat to 72C for 60 sec (elongation).

Question 8

What are the substrates of DNA replication?

Answer 8

Primer (RNA), bases.

Question 9

What is DNA catalyzed by?

Answer 9

DNA polymerase.