DNA Structure & Replication

Question 1

What does DNA polymerase require to bind?

Answer 1

A free 3’ hydroxyl

Question 2

What provides the 3’ hydroxyl end DNA polymerase needs to bind?

Answer 2

An RNA primer by binding to the template once the strands are unwound

Question 3

In what form is each nucleotide first added?

Answer 3

In a triphosphate form

Question 4

What happens when nucleotides are added in the triphosphate form?

Answer 4

The triphosphate diffuses into the active site, a phosphodiester bond forms between the 3’ hydroxyl group of the primer and the 5’ phosphate group releasing a pyrophosphate

Question 5

What provides the driving force of DNA replication?

Answer 5

The hydrolysis of pyrophosphate

Question 6

What us required to add each nucleotide to the growing chain?

Answer 6

2 Hugh energy phosphate bonds