DNA Technology

Question 1

DNA library

Answer 1

knowing the DNA sequence based on proteins

Question 2

reverse transcriptase

Answer 2

takes mRNA and makes complementary DNA (cDNA) which is single-stranded (ss)

Question 3

DNA polymerase

Answer 3

generates double-stranded DNA from ssDNA

Question 4

gene amplification

Answer 4

DNA injected into a plasmid or virus cloning vector, which infects a bacteria and creates lots of dsDNA

• the use of bacteria resistant to abx to produce a gene of interest

Question 5

DNA cloning

Answer 5

Produce identical copies of DNA

1. restriction enzyme “cut” DNA
2. DNA ligase ties the strand of DNA into a plasmid
3. apply a gene for antibiotic resistance
4. heat shock allows the bacteria to take up the plasmid

Question 6

cloning and cDNA

Answer 6

isolate the mRNA, convert it into DNA using reverse transcriptase

Question 7

Southern blot

Answer 7

visualize a specific sequence of DNA

1. cleave DNA
2. gel electrophoresis
3. gel -> filter
4. take the filter and expose to radio-labeled piece of DNA
5. expose to X-ray