genogroup of CSFV in the country
G2, G3
Indirect ELISA: test serum is placed in wells of _____
polystyrene microtitre plates
is the antiglobulin linked to an enzyme
conjugate
ELISA Color reaction is visualised and measured with
spectrophotometer
antigen is pre-adsorbed at the bottom of the well T.F
true
how many nm is used to determine adsorbance
405 nm
used to
measure optical density (OD) or color intensity of
the reaction for each well
Spectrophotometer
test validation:
neg control: <0.1
pos control: >0.1
formula for S/P ration:
test sample OD - OD neg control/ OD pos control - OD neg control =
S/P >0.25 =
S/P <0.25 =
pos
neg
it is recommended to have no more than __ freeze-thaw cycle
3-5