DSP examples

Question 1

CAR-T cells

Answer 1

1. Leukapheresis of blood to remove white blood cells
2. isolation of T-cells ( affinity chromatography, Magnetic flow cytometry..)
3. T-cell activation using Dynabeads whih transfer genetic material into cell
4. T-cel transduction
5. modified cell expansion
6. formultaion and bead removal by magnetic flow cytometry
7. release testing
8. infusion into the patient

Question 2

how does Herceptin work

Answer 2

a monoclonal antibody which targets cells with lots of HER-2 receptors, stops growth signal and signals immune response

Question 3

Herceptin purification

Answer 3

1. filtration
2. affinity chromatography to remove endotoxins
3. Cation chromatography to remove aggregates and inpurities
4. Anion chromatography to remove DNA, retroviruses, endo toxins
5. hydrophobic interaction chromatography
6. formulation
7. filtration
8. freeze dry and storage

Question 4

What is a biosimilar

Answer 4

a drug of biologic origin designed to have active prperties similar to those of an approved biologic origin drug

Question 5

HGH purification

Answer 5

1. centrifuge to settle cells
2. sonicate
3. centrifuge to disrupt cells
4. affinity chromatography
5. anion chromatography
6. trk cleavage ( tag used in affinity chromatography)
7. ultrafiltration

Question 6

How can HGH be analysed?

Answer 6

MAss spec SDS page, western blot