1
Q
Transformation efficiency
A
Total number of colonies growing on the LB/amp per μg of plasmid DNA used for the transformation
2
Q
Initial amount or mass of plasmid DNA
A
Total amount of DNA (μg) = concentration (μg/μl) X volume (μl)
3
Q
Fraction of DNA solution spread
A
Volume of solution spread on plate divided by total amount in tube = fraction spread
4
Q
Mass of DNA spread on the experimental plate
A
Total mass of DNA [μg] X fraction spread = Mass of DNA spread
5
Q
Number of colonies per μg of plasmid DNA
A
Number of colonies observed divided by mass of DNA spread = Transformation efficiency
Bio Mod 6
flashcards
Decks in class (19)
# Cards
-
6.1.1 - Mutagen operations9
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6.1.2 - Point and Chromosomal Mutations9
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Chromosomal abnormalities case studies2
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6.1.3 - Somatic v Germline mutations2
-
6.1.4 - Coding v Non-coding DNA segments11
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6.1.5 - Causes of Genetic Variation2
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6.1.6 - Mutation, gene flow, and genetic drift on changing gene pools9
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6.2 - Biotechnology23
-
DNA Technologies12
-
Depth study GMO and Transgenic organisms13
-
Depth study Lesson 57
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6.4 -Recombinant DNA for Transgenic Organism10
-
Biotechnology case studies5
-
6.3 Cloning8
-
6.3.1-2 - AI and Genetic Change7
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E. coli calculation formulas5
-
Experiment - E.Coli19
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Sterilisation Techniques8
-
6.2.1 - Bioethics8
