EMT

Question 1

Features of epithelial cells

Answer 1

• adherence junctions
• tight junctions
• desmosomes
• apico-basal polarity
• stationary
• cytokeratins
• cortical actins
• E-cadherens

Question 2

Features of mesenchymal cells

Answer 2

• no junctions
• focal adhesions
• ECM deposition
• Anokis resistant
• motile and invasive
• front-back polarity
• Vimentin/SMA
• FSP-1
• FN, CSPG

Question 3

What is Vectinnin and fibrocin

Answer 3

Markers of mesenchymal cells

Question 4

What are snail and twist

Answer 4

Transcription factors that block transcription for E-cadherin. Triggering the transition the mesenchymal phenotype.

Question 5

What is TGFB

Answer 5

Signalling molecule that induces EMT

Question 6

What are the stages of EMT in development (in peripheral nervous system)

Answer 6

• Development of peripheral nervous system from neural tube
  ○ Neural tube - tightly bound epithelial cells
• Epithelial cells form neural crest cells
  ○ Have the ability to migrate
• These can then migrate to areas to differentiate to form the peripheral nervous system