H2S positive: black production
“Curious Engineers Produce Smoke.”
Citrobacter Freundii
Edwardsiella tarda
Proteus sp.
Salmonella enterica
Non-motile: No Flagella, SIM test M: negative
“ lazy Kings Sit”
Klebsiella Pneumoniae
Shigella sp.
Nonmotile at 37 degrees Celsius
Yersinia pestis
Proteus sp.
Providencia sp.
Morganella morganii
“Pretty People Make Green”
Confirmation test is PDA-positive (Phenylalanine Deaminase–positive) organisms of the Enterobacterales family
Enterobacter aerogenes (now Klebsiella aerogenes)
Klebsiella species
Serratia marcescens: DNase +
“Enter Klebs Spit Fire”
Voges–Proskauer Positive
Clinical Implication: UTI
Most Common: Escherichia Coli
Proteus sp.
Providencia sp.
Morganella morganii
Clinical Implication: Gastroenteritis
Providencia sp.
Clinical Implication: Wound infection
Edwardsiella tarda
Clinical Implication: Nosocomial Infections
SECK
Serratia
Enterbactor aerogenes
Citrobacter freundii
Klesibella pneumoniae
Most common: Citrobacter freundii & Enterbactor aerogenes
Pneumonia in hospitalized patients
Klebsiella pneumoniae
Wound infection from burns
Serratia marcescens
bacillary dysentery: acute infection of the gastrointestinal tract
Shigella sp.
Bacteremia, Typhoid fever
Salmonella sp.
SEECK
“seeck and you shall see pink”
LF- pink growth
Serratia
Escherichia Coli
Enterbactor aerogenes
Citrobacter freundii
Klesibella pneumonaie
MESS PP
“MESSing PP-pink”
NLF
Morganella Morganii
Edwardsiella tarda
Salmonella enterica
Shigella spp.
Proteus sp.
Providencia sp.
Using only indole test to differentiate Proteus mirabilis and proteus vulgaris
Indole Test
Proteus mirabilis (-)
Proteus vulgaris (+)
Oxidase Negative: no blue
Catalase Positive: Bubbles
Grows on Mac
GNR Non hemolytic
All Enterobacterales are
Indole (+): Red/ pink color 🔴
“I MEEPP the bloody floor”
Morganella morganii
Escherichia coli
Edwardsiella tarda
Providencia sp.
Proteus vulgaris
Citrate positive
“Purely Pretty Colored Skes” 🔵
Providencia sp.
Proteus vulgaris
Citrobacter freundii
Salmonella enterica
Klebsiella pneumoniae
Enterobacter aerogenes
Serratia marcescens
Very mucoid colonies run together
Klebsiella pneumoniae
Swarming growth
Proteus mirabilis
Distinct indole odor
Escherichia coli
Slow lactose fermenter
Serratia marcescens
Enterobacter Aerogenes
Red wind pigment (prodigiosin)
Serratia marcescens