Enzyme

Question 1

Enzyme regulation
Must be regulated so that the proper level of ________ are produced at all times and places . We do this to control the conc of product produced.

Answer 1

product or concentration of products of

Question 2

Regulation/controls of enzymatic activity occurs in several ways

1)bilosynthesis at the genetic level:
By ______ in which some control mechanism triggers synthesis of proteins _____
2)covalent modification: synthesized enzyme can be modified covalently. It’s when you add a _______ molecule(like co2,amino group,etc)
3)regulatory enzyme:one enzyme is regulated by _____
4)feedback inhibition: when the enzyme forms ______, the conc of the product being formed in relation to the substrate can regulate the reaction

Answer 2

Enzyme induction; ribosomally

covalent

another enzyme.

ES complex

Question 3

______ inhibits most microsomal enzymes reaction but it also inhibits the ______ of procaine which is definitely a non microsomal enzyme mediated reaction

Answer 3

SKF525A

hydrolysis

Procaine

Question 4

Mechanism of SKF525A is (competitive or non competitive?) in most cases and also (competitive or non competitive?)

It is certain that SKF525A binds to microsomal enzymes and inhibit them both _____ and ____

Answer 4

competitive

non competitive

in vivo and in vitro

Question 5

Piperonyl butoxide:

• acts as an alternative substrate meaning that its mechanism is ____ and more active ______ than _____
• it also controls the synthesis of ______

Answer 5

competitive

in vivo than in vitro

cytP450

Question 6

EFFECTS OF ENZYME INHIBITION

• some inhibitors are used clinically
• e.g _____ inhibits xanthin oxidase and the metabolism of its compounds thereby increasing the ______ efficacy of the drug(———)

Answer 6

Aluporinol

anti cancer

6mercapto purine

Question 7

disulphiram allows _______ to accumulate after _____ ingestion leading to a consequent toxic effect

Answer 7

acetaldehyde

alcohol

Question 8

ATP As energy source

• ATP to ___ releases energy
• one P removed, releases energy of up to __Kilocalories per mole /____ kilojoules per mole
• powers all reactions that take place in the cell
• ADP can be converted to ATP so that energy can be available for other cellular reactions

Answer 8

ADP

7. 3
8. 6

Question 9

DNA synthesis

• _______ is formed from ATP
• _______ is former form CTP
• and so on and on
• before being put into mRNA

-but for dna, ATP has to be converted to _____ first before joining the dna strand

Answer 9

adenosine

cytosine

dATP