1
Q
what does exonuc III do?
A
from e coli, cut single bases off of the 3’ end
2
Q
what does exonuc V do?
A
can remove bases from both the 5’ and 3’ ends (and has some endo activity as well)
3
Q
T7 Exonuclease
A
can remove single bases from the 5’ end
4
Q
S1 endonuclease purpose?
A
from an Aspergillus fungus will cut only single-stranded DNA
5
Q
DNase I purpose?
A
from cow pancreas will cleave dsDNA, ssDNA and only the DNA in a DNA:RNA hybrid
6
Q
RNAse H purpose?
A
will cut only RNA in a DNA:RNA hybrid
7
Q
T7 Endonuclease I
A
will cut at structural oddities like mismatched or cruciform DNA
