estruch flashc Flashcards

(213 cards)

1
Q

monomer

A

the smaller units from which larger molecules are made

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2
Q

polymer

A

molecules are made from a large number of monomers joined together

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3
Q

disaccharide

A

formed by the condensation of two monosaccharides

held together by a glycosidic bond

e.g: maltose, sucrose, lactose

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4
Q

monosaccharide

A

monomers from which larger carbohydrates are made

e.g: glucose, fructose, galactose

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5
Q

polysaccharide

A

formed by the condensation of many glucose units

held by glycosidic bonds

e.g: starch, glycogen, cellulose

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6
Q

cellulose

A

polysaccharide in plant cell walls

formed by the condensation of beta glucose monomers

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7
Q

condensation reaction

A

a reaction that joins two molecules togethers

with the formation of a chemical bond

involves the elimination of a molecule of water

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7
Q

glycogen

A

polysaccharide in animals

formed by the condensation of alpha glucose monomers

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8
Q

starch

A

polysaccharide in plants

formed by the condensation of alpha glucose monomers

contains two polymers - amylose and amylopectin

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9
Q

amylose

A

polysaccharide in starch

made of alpha glucose monomers

joined by 1,4 glycosidic bonds

coils to form a helix

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9
Q

glycosidic bond

A

C-O-C link

between two sugar molecules

formed by a condensation reaction

it is a covalent bond

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10
Q

amylopectin

A

polysaccharide in starch

made of alpha glucose

joined by a 1,4 and 1,6 glycosidic bonds

branched structure

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11
Q

hydrolysis reaction

A

a reaction that breaks a chemical bond

between two molecules

involves the use of a water molecule

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12
Q

fibrils

A

long, straight chains of beta glucose monomers

held together by many hydrogen bonds

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13
Q

triglyceride

A

formed by the condensation of one molecule of glycerol and three molecules of fatty acids

forming 3 ester bonds

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14
Q

phospholipid

A

formed by the condensation of one molecule of glycerol and two molecules of fatty acid

help by 2 ester bonds

a phosphate group is attached to the glycerol

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15
Q

induced-fit model

A

the enzyme active site is not initially complementary to the substrate

the active site moulds around the substrate

this puts tension on bonds

lowers the activation energy

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16
Q

competitive inhibitor

A

a molecule that is a similar shape to the substrate

binds to active site

prevents enzyme-substrate complexes from forming

forms enzyme-inhibitor complex

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17
Q

non-competitive inhibitor

A

a molecule that binds to an enzyme at the allosteric site

causing the active site to change shape permanently

preventing enzyme-substrate complexes from forming

forms enzyme-inhibitor complex

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18
Q

primary structure of proteins

A

sequence of amino acids in a polypeptide chain

peptide bonds between amino acid

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19
Q

secondary structure of proteins

A

the folding or coiling of polypeptide

to create a beta pleated sheet or an alpha helix

held in place by hydrogen bonds

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20
Q

tertiary structure of protein

A

the further folding

to create a unique 3D shape

held in place by hydrogen, ionic and sometimes disulfide bonds

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21
Q

quaternary structure of protein

A

more than one polypeptide chain in a protein

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22
Q

peptide bond

A

covalent bond joining amino acids together in proteins

C-N link between an amine and carboxyl group of two different amino acid molecules

formed by a condensation reaction

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23
what is the effect of temperature on enzyme-controlled reaction?
at low temperature, there is not enough KE for successful collisions between the enzyme and substrate at too high temperature, enzymes denature; active site changes shape and enzyme-substrate complexes cannot form
24
what is the effect of pH on enzyme-controlled reaction?
too high or too low a pH will interfere witht he charges in the amino acids in the active site this breaks ionic and hydrogen bonds holding tertiary structure in place hence, active site changes shape and enzymes denatures different enzymes have a different optimal pH
25
what is the effect of substrate concentration on enzyme-controlled reaction?
at low substrate conc, there will be fewer collisions between the enzyme and substrate at high substrate conc, the rate plateaus bc all the enzyme active sites are saturated
26
what is the effect of enzyme concentration on enzyme-controlled reaction?
at low enzyme conc, there will be fewer collisions between enzyme and substrate at high enzyme conc, the rate plateaus bc there are more enzymes than the substrate, so there are empty active sites
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hydrophilic
the ability to mix, interact or attract water e.g: head region on a phospholipid
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ester bond
COO chemical bond formed between glycerol and fatty acids
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hydrophobic
the tendency to repel and not mix with water e.g: tail region on a phospholipid
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galactose
the monosaccharide that reacts with glucose to form lactose
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glucose
monosaccharide that exists as two isomers alpha glucose and beta glucose
30
fructose
monosaccharide that reacts with glucose to form sucrose
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isomer
molecules with the same molecular formula but the atoms are arranged differently
32
maltose
disaccharide formed by the condensation of two glucose molecules
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lactose
disaccharide formed by the condensation of a glucose molecule and a galactose molecule
34
sucrose
disaccharide formed by the condensation of a glucose molecule and a fructose molecule it is a non-reducing sugar
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polypeptide
polymer chain of a protein made up of amino acids bonded together by peptide bonds via condensation reactions
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amino acid
the monomer of a protein formed from C, H, O, N contain a carboxyl group, amino group, and H, and an R group
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amine group
NH2 group found on amino acids
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carboxyl group
COOH group made up of a C with hydroxyl (OH) and carbonyl (C=O) group bonded to it found in amino acids and fatty acids
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R group on amino acids
the variable group the part of each of the 20 amino acids that is different
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alpha helix
a secondary structure in proteins the polypeptide chain is coiled up and held in place by hydrogen bonds
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beta pleated sheet
a secondary structure in proteins the polypeptide chain folded into pleated sheets held in place by hydrogen bonds
42
hydrogen bonds
weak bond forms between H and O in many biological molecules, e.g: proteins, DNA and tRNA
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ionic bonds
a bond that forms between the R group of different amino acids in the tertiary structure of proteins
44
disulfide bonds
a strong covalent bond between two sulfur atoms in the R groups of different amino acids in the tertiary structure of proteins
45
active site
unique-shaped parts of an enzyme that the substrate binds to
46
activation energy
the minimum amount of energy required for a reaction to occur
47
enzyme-subrate complex
forms when an enzyme and substrate collide and bind resulting in a lowered activation energy
48
unsaturated fatty acid
a long hydrocarbon chain with a carboxyl group at one end at lease one double bond between carbon atoms
48
denature
when the active site changes shape so the substrate can no longer bind, and no enzyme-subsrate complexes form
49
enzyme-inhibitor complex
the structure that forms when an enzyme and inhibitor collide and bind prevents enzyme-subsrate complexes from forming
50
saturated fatty acid
a long hydrocarbon chain with a carboxyl group at one end only single bonds between carbon atoms
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polar molecule
a molecule that has an uneven distribution of charge
52
describe the structure of a phospholipid bilayer
a phospholipid has: - a hydrophilic (polar) head that is attracted to water - two hydrophobic (non-polar) tails that repel water in a bilayer, phospholipids form two layers, with the heads on the outside and the tails on the inside
53
plasma membrane
phospholipid bilayer cell surface membranes and organelle membranes
54
reducing sugar
sugars that can reduce Cu2+ ions in Benedict's reagent to form Cu+ ions in the form of copper (I) oxide which forms a brick-red precipitate
55
test for reducing sugar?
add Benedict's reagent heat observe green/yellow/orange/brick red precipitate
56
how does the structure of a triglyceride relate to its function?
high ratio of C-H bonds to carbon atoms = lots of energy released when broken high hydrogen to oxygen ratio = release water when oxidised (metabolic water source) large, non-polar = insoluble in water, doesn't affect water potential low mass to energy ratio = energy-rich storage molecule
57
how does the structure of a phospholipid relate to its function?
phospholipids have two charged regions, so they are polar in water, they are positioned so that the heads are exposed to water and the tails are not this forms a phospholipid bilayer, which makes up the plasma membrane around cells
58
how does the structure of a triglyceride and phospholipid differ?
triglyceride: 1 glycerol + 3 fatty acids phospholipids: 1 glycerol + 2 fatty acids + 1 phosphate group triglycerides are non-polar and hydrophobic phospholipids have a polar (hydrophilic) head and non-polar (hydrophobic) tails
59
what is the difference between saturated and unsaturated fatty acid?
a saturated fatty acid has no double bonds between carbon atoms whereas unsaturated fatty acids have at least one double bond between carbon atoms
60
non-reducing sugar
a sugar unable to reduce Cu2+ glycosidic bond must be hydrolysed to expose the reducing group e.g: sucrose
61
test for non-reducing sugar
(following a negative Benedict's test) boil sample in acid and then neutralise with alkaline add Benedict's reagent and heat observe brick red precipitate
62
test for starch
add iodine solution turns blue/black
63
test for lipids
add ethanol then add water shake white emulsion forms
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test for protein
add biuret reagent turns purple/lilac
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nucleotide
monomer of DNA and RNA contains a pentose sugar, a phosphate group and a nitrogenous base
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nitrogenous base
part of a nucleotide adenine, guanine, cytosine, thymine and uracil
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DNA nucleotide
monomer of DNA contains a deoxyribose sugar, a phosphate group and a nitrogenous base
64
polynucleotide
DNA/RNA polymer many nucleotides joined together via a condensation reaction joined by phosphodiester bonds
64
phosphodiester bond
bond joining two adjacent nucleotides together formed via condensation reaction forms between a phosphate group and the pentose sugar
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complementary base pairs
the base pairs that align opposite each other and form hydrogen bonds A and T (DNA) A and U (RNA) C and G
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ribose
pentose sugar found in RNA nucleotide and ATP
67
uracil
nitrogenous base found in RNA instead of thymine
68
what is the role of DNA and RNA in cells?
DNA holds genetic info in all living cells RNA transfers genetic info from DNA to the ribosomes for protein synthesis
69
what are ribosomes formed from?
RNA proteins
70
describe the structure of a DNA molecule
nucleotide monomer DNA is a polymer double helix made of two polynucleotide chains held together by hydrogen bonds between specific complementary base pairs
71
DNA template strand
a DNA strand that is used to make a new copy from both DNA strands in the double helix are used as templates in DNA replication
72
DNA polymerase
an enzyme in DNA replication joins together adjacent nucleotides
73
semi-conservative replication
DNA replication is semi-conservative replication each new DNA molecule contains one original (parental) strand and one newly synthesised strand
74
DNA helicase
enzyme that breaks hydrogen bonds between the chains of DNA in a double helix causes the two strands to separate involved in DNA replication
75
large latent heat of vaporisation
a lot of energy is required to convert water from its liquid state to a gaseous state this is due to the hydrogen bonds between molecules, as energy is needed to break these to turn it into a gas means water can provide a cooling effect
76
high specific heat capacity
a lot of energy is required to raise the temperature of water because some of the heat energy is used to break the hydrogen bonds between water molecules important so water can act as a temperature buffer
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metabolite
water is involved in many reactions such as photosynthesis, hydrolysis, and condensation reactions
78
list the 5 main properties/roles of water
large latent heat of vaporisation high specific heat capacity metabolite solvent strong cohesion
79
solvent
water is a good solvent meaning many substances dissolve in it polar (charged) molecules dissolve readily in water, due to the fact water is polar
80
strong cohesion
water molecules 'stick' together due to hydrogen bonds results in water moving up the xylem as a continuous column of water provides surface tension, creating a habitat on the surface of the water for small invertebrates
80
ATP synthase
enzymes that catalyses the synthesis of ATP from ADP + Pi
81
ATP hydrolase
enzyme that catalyses the hydrolysis of ATP into ADP + Pi
82
phosphorylation
the addition of a phosphate group to a molecule making the molecule more reactive/ it gains energy
83
dipeptide
two amino acids joined together by a peptide bond formed by a condensation reaction
83
RNA nucleotide
monomer of RNA composed of a phosphate group, ribose and a nitrogenous base has the base uracil, instead of thymine
83
structure of water
water is a polar molecule the oxygen atom is slightly negative the hydrogen atoms are slightly positive
84
role of hydrogen ions
determine the pH the more hydrogen ions, the more acidic the conditions are an important role in chemiosmosis in respiration and photosynthesis
85
role of iron ions
a compound haemoglobin involved in oxygen transport
86
role of sodium ions
involved in co-transport for absorption of glucose and amino acids in the ileum
87
role of phosphate ions
as a component of DNA, RNA and ATP phosphodiester bond in DNA and RNA forms between the phosphate group and the pentose sugar
88
fatty acid structure
a carboxyl group and a long hydrocarbon chain can be saturated or unsaturated
89
describe how an ester bond forms?
a condensation reaction between glycerol and a fatty acid (RCOOH)
90
what is the R group of a fatty acid?
the hydrocarbon chain it can be saturated or unsaturated
91
explain the arrangement of phospholipids in a cell-surface membrane
in water, phospholipids form a bilayer hydrophobic (fatty acid) tails point away, as they are repelled from water hydrophilic (phosphate) heads point to and are attracted to water
92
describe DNA replication
DNA helicase unwinds DNA double helix and breaks its hydrogen bonds both strands act as template strands free floating DNA nucleotides are attracted to their complementary base pairs on exposed strands, with which they form hydrogen bonds DNA polymerase catalyses the formation of phosphodiester bonds between adjacent nucleotides
93
describe the structure of an RNA molecule
relatively short single polynucleotide chain contains the base uracil (instead of thymine), and the sugar ribose (instead of deoxyribose)
94
ATP structure
a nucleotide derivative formed from ribose, adenine, and 3 phosphate groups
95
state 5 important properties of water
metabolite solvent high specific heat capacity large latent heat of vaporisation strong cohesion between water molecules
96
what are the roles of inorganic ions in the body?
occur in cytoplasm and body fluids in varying concentrations H+ ions: affect pH Fe2+ ions: component of haemoglobin Na+ ions: involved in co-transport of glucose and amino acids PO4^3- ions: found in DNA and ATP (form phosphodiester bonds)
97
true or false: 'all living organisms contain a wide variety of carbon-based compounds that function in entirely different ways'
false all organisms contain only a few groups of carbon-based compounds that interact in similar ways. this provides indirect evidence for evolution
98
true or false: 'glucose, galactose and fructose are all monosaccharides'
true
99
true or false: 'glycogen and cellulose are both formed from alpha glucose'
false glycogen is formed from alpha glucose, but cellulose is formed from beta glucose
100
true or false: 'phospholipids contain 3 fatty acid chains to attached to glycerol'
false phospholipids contain 2 fatty acid chains; the third is replaced by a phosphate group
101
true or false: 'a peptide bond is formed by a condensation reaction between two amino acids'
true
102
true or false: 'the induced-fit model suggests that the active site of an enzyme is always exactly complementary to the substrate'
false in the induced-fit model, the active site moulds around the substrate, becoming complementary as it binds
103
true or false: 'DNA is a double-stranded helix held together by hydrogen bonds between complementary base pairs'
true
104
true or false: 'water has a low latent heat of vaporisation, which helps organisms cool down easily without loosing too much water'
false water has a high latent heat of vaporisation, so it provides a cooling effect with minimal water loss
105
nucleus structure
nuclear pores, nucleolus, DNA and nuclear envelope
105
cell membrane function
selectively permeable barrier controls passage of substances in and out the cell barrier between internal and external cell environments
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cell membrane structure
phospholipid bilayer with embedded intrinsic & extrinsic proteins
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nucleus function
site of transcription and pre-mRNA splicing - mRNA production site of DNA replication nucleolus makes ribosomes nuclear pores allows movement of substances to/from cytoplasm
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mitochondria structure
double membrane with inner membrane folded into cristae 70S ribosomes in matrix small, circular DNA enzymes in matrix
108
mitochondria function
site of aerobic respiration produces ATP
108
chloroplast structure
thylakoid membranes stacked to form grana, lined by lamellae stroma contains enzymes contains starch granules, small circular DNA and 70S ribosomes
108
chloroplast function
chlorophyll absorbs light for photosynthesis to produce organic molecules (glucose)
109
lysosome function
contains digestive enzymes e.g: lysozymes to hydrolyse pathogens/cell waste products
109
lysosome structur
type of golgi vesicle containing digestive enzymes
109
golgi apparatus structure
fluid-filled, membrane-bound sacs (horseshoe shaped) vesicles at edge
109
golgi apparatus function
modifies proteins received from rough endoplasmic reticulum (rER) packages them into vesicles to transport to cell membrane for exocytosis makes lysosomes
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what organisms contain chloroplasts?
plants algae
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smooth endoplasmic reticulum function
synthesises and processes lipids
110
rough endoplasmic reticulum function
site of protein synthesis folds polypeptides to secondary and tertiary structures packaging into vesicles to transport to golgi
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ribosome sturcture
small and large subunit made of protein and rRNA free floating in cytoplasm and bound to rER 70S in prokaryotes, mitochondria and chloroplasts 80S in eukaryotes
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cell wall function
provides structural strength, rigidity and support to cell helps resist osmotic pressures
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ribosome function
site of translation in protein synthesis
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rough endoplasmic reticulum structure
system of membranes with bound ribosomes continuous with nucleus
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smooth endoplasmic reticulum structure
system of membranes with no bound ribosomes
115
cell wall structure
in plants, fungal and bacterial cells plants = made of microfibrils of cellulose fungi = made of chitin bacteria = murein/ peptidoglycan
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cell vacuole structure
fluid-filled surrounded by a single membrane called a tonoplast
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contrast prokaryotic and eukaryotic cells
prokaryotic cells are smaller prokaryotes have no membrane bound organelles prokaryotes have smaller 70S ribosomes prokaryotes have no nucleus - circular DNA not associated with histones prokaryotic cell wall made of murein, instead of cellulose/ chitin
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occasional features of prokaryotes?
plasmids = loops of DNA (slime) capsule surrounding cell wall = helps agglutination + adds protection flagella = for movement
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cell vacuole function
makes cell turgid - structural support temporary store of sugars, amino acids coloured pigments attract pollinators
120
protein channels
tubes filled with water, enabling water-soluble ions to pass through the membrane selective channel proteins only open in the presence of certain ions when they bind to the protein
120
protein carriers
bind with a molecule (e.g: glucose) which causes a change in the shape of the protein this change in shape enables the molecule to be released, to the other side of the membrane
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features of virsuses
non-living and acellular contains genetic material, capsid and attachment proteins some (HIV) contain a lipid envelope + enzymes (reverse transcriptase)
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3 types of microscopes
optical (light) microscopes scanning electron microscopes (SEM) transmission electron microscopes (TEM)
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magnification
how many times larger the image is compared to the object calculated by equation: magnification = image size/ actual size
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resolution
the minimum distance between two objects in which they can still be viewed as separate determined by wavelength of light (for optical microscopes) or electrons (for electron microscopes)
125
optical microscopes
beam of light used to create image glass lens used for focusing 2D coloured image produced
125
what wavelength is longer: visible light or electrons?
visible light has a longer wavelength than electrons
126
evaluate optical microscopes
- poorer resolution, as long wavelength of light - small organelles not visible - lower magnification + can view living samples + simple staining method + vacuum not required
127
transmission electron microscopes
beam of electrons passes through the sample used to create an image focused using electromagnets 2D, black & white image produced can see internal ultrastructure of cell structures absorb electrons and appear dark
128
evaluate TEMs
+ high resolving power + high magnification - extremely thin specimens required - complex staining method - specimen must be dead - vacuum required
129
scanning electron microscopes
beam of electrons pass across sample used to create image focused using electromagnets 3D, black and white image produced electrons scattered across specimen producing image
130
evaluate SEMs
+ high resolving power + high magnification + thick specimens usable - complex staining method - specimen must be dead - vacuum required
131
why calibrate eyepiece graticule?
calibration of the eyepiece is required each time the objective lens is changed calibration to work out the distance between each division at that magnification
132
purpose of cell fractionation
break open cells & remove debris so organelles can be studied
133
homogenisation
process by which cells are broken open, so organelles are free to be separated done using homogeniser (blender)
134
homogenisation conditions
cold = reduces enzyme activity, preventing organelle digestion isotonic = prevents movement of water by osmosis - no bursting/shrivelling of organelles buffered = resist pH changes, preventing organelle + enzyme damage
134
differential centrifugation
supernatant first out (spun at lowest speed) is most dense = nuclei spun at higher speeds nuclei --> chloroplasts --> mitochondria --> lysosomes --> rER/sER --> ribosomes (least dense)
135
ultra-centrifugation
homogenate solution filtered of to remove cell debris solution placed in a centrifuge, which spins at a lower speed initially then increasing faster speeds, to separate organelles according to their density
136
binary fission
involves circular DNA & plasmids replicating cytokinesis creates two daughter nuclei each daughter cell has one copy of circular DNA and a variable number of plasmids
137
cell cycle
1) interphase (G1, S, G2) 2) nuclear division = mitosis or meiosis 3) cytokinesis
138
interphase
longest stage in the cell cycle when DNA replicates (s-phase) and organelles duplicate while cell grows (G1 & G2 - phase) DNA replicates and appears as two sister chromatids held by centromere
139
mitosis
one round of cell division two diploid, genetically identical daughter cells growth and repair (e.g: clonal expansion) comprised of prophase, metaphase, anaphase, and telophase
140
prophase
chromosomes condense and become visible nuclear envelope disintegrates in animals, centrioles separate & spindle fibre structure forms
140
metaphase
chromosomes align along equator of cell spindle fibres released from poles, now attach to centromere and chromatid
141
anaphase
spindle fibre contracts (using ATP) to pull chromatids, centromere first, towards opposite poles of cell centromere divides in two
142
telophase
chromosomes at each pole become longer and thinner again spindle fibre disintegrate + nucleus reforms
143
mitotic index
used to determine proportion of cells undergoing mitosis calculated as a % or a decimal mitotic index = (number of cells in mitosis) / (total number of cells) x 100 (for %)
143
fluid mosaic model
describes the lateral movement of membranes with scattered embedded intrinsic and extrinsic proteins membrane contains glycoproteins, glycolipids, phospholipids and cholesterol
144
cholesterol
present in eukaryotic organisms to restrict lateral movement of membranes adds rigidity to membrane = resistance to high temp & prevents water + dissolved ions leaking out
144
facilitated diffusion
passive process using protein channels/ carriers down the conc gradient used for ions and polar molecules, e.g: sodium ions and large molecules, e.g: glucose
145
phospholipids in membrane
phospholipids align as a bilayer hydrophilic heads are attracted to water hydrophobic tails repelled by water
145
selectively permeable membrane
molecules must have specific properties to pass through plasma membrane: - lipid soluble (hormones, e.g: oestrogen) - very small molecules - non-polar molecules (oxygen)
145
simple diffusion
net movement of molecules from an area of higher conc to an area of lower conc until equilibrium is reached passive
146
osmosis
net movement of water from an area of higher water potential to an area of lower (more negative) water potential across a partially permeable membrane
146
water potential
the pressure created by water molecules measured in kPa and represented by symbol Ψ pure water has a water potential is 0kPa the more negative the water potential, the more solute must be dissolved
147
hypertonic solution
when the water potential of a solution is more negative then the cell water moves out of the cell by osmosis both animal and plant cells will shrink ad shrivel
147
hypotonic solution
when the water potential of a solution is more positive (close to zero) than the cell water moves into the cell by osmosis animal cells will lyse (burst) plant cells will become turgid
148
isotonic
when the water potential of the surrounding solution is the same as the water potential inside the cell no net movement in water cells would remain the same mass
149
co-transport
the movement of two substances across a membrane together, when one is unable to cross the membrane itself involves a co-transport protein e.g: absorption of glucose. amino acids from lumen of intestine
149
role of carrier protein in active transport
when molecules bind to the receptor, ATP will bind to protein on inside of membrane, and is hydrolysed to ADP + Pi protein changes shape and opens inside membrane
149
active transport
the movement of ions and molecules from an area of lower conc to an area of higher conc, using ATP and carrier proteins carrier proteins act as selective pumps to move substances
149
what molecules can lymphocytes identify?
- pathogen (bacteria, fungi, viruses) - cells from other organisms of the same species (transplants) - abnormal body cells (tumour cells) - toxins (released from bacteria)
150
antigens
proteins on the cell-surface membrane triggers an immune response when detected by lymphocytes
151
antigenic variability
when pathogenic
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