Exam 2 Flashcards

(168 cards)

1
Q

What is a concentration
gradient?

A

difference in concentration of a dissolved substance across a space.

Substances tend to move from a high to low concentration.

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2
Q

What is Diffusion?

A

(net) movement
of molecules from an area
of greater concentration to
an area of lower
concentration

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3
Q

Does diffusion require energy?

A

no

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4
Q

What is Osmosis?

A

Diffusion of water (or another solvent) from area of ↑concentration
(usually in comparison to ions or large molecules) to area of ↓concentration

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5
Q

What is hypertonic?

A

Comparative term designating a solution that, if outside a cell or vesicle, results in the LOSS of water and SHRINKAGE of the membrane-bound structure. This solution has a greater solute concentration than the solution on the other side of the membrane.

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6
Q

What is hypotonic?

A

Comparative term designating a solution that, if outside a cell or vesicle, results in the uptake of water and SWELLING or even BUSTING of the membrane-bound structure. This solution has a lower solute concentration than the solution on the other side of the membrane.

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7
Q

What is isotonic?

A

Comparative term designating a solution that, if inside a cell or vesicle, results in no net uptake or loss of water and thus no effect on the volume of the membrane-bound structure. This solution has the same solute concentration as the solution on the other side of the membrane.

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8
Q

What kinds of molecules does a semipermeable membrane let across?

A

The plasma membrane is a semipermeable membrane that only easily lets
across small, non-polar molecules (and ions/molecules that have protein
channels/carriers/pumps)
*

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9
Q

Why does water move toward areas of higher solute (ion) concentration in cells?

A

ions cross the cell membrane much more slowly than water, so water moves by osmosis toward the side with higher solute (lower water) concentration.

(water moves instead of ions bc its easier for it)

imagine:
Inside the cell: lots of ions (salty)

Outside the cell: fewer ions (less salty)

The ions can’t move out easily to balance things.

So instead…

👉 Water moves toward the side with more ions

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10
Q

What is Facilitated diffusion?

A

movement of ions/molecules down their concentration gradient through a transport protein embedded in membrane

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11
Q

Does Facilitated diffusion require energy?

A

no

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12
Q

What is Active transport?

A

The movement of ions or molecules across a membrane in a single direction, often against a gradient. Requires energy (e.g., from hydrolysis of ATP) and assistance of a transport protein (e.g., pump).

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13
Q

Does Active Transport require energy?

A

Requires energy (e.g., from hydrolysis of ATP) and assistance of a transport protein (e.g., pump).

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14
Q

What can Facilitated diffusion be accomplished through?

A
  1. channel proteins
  2. carrier proteins
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15
Q

What is an electrochemical gradient?

A

high to low concentration; like to unlike charge

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16
Q

Why is cell size limited?

A

Because diffusion and the surface area-to-volume ratio limit how efficiently nutrients enter and wastes leave the cell.

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17
Q

How does surface area-to-volume ratio affect cells?

A

As a cell gets bigger, its volume increases faster than its surface area, making exchange across the plasma membrane less efficient.

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18
Q

What happens to nutrient and waste exchange as a cell gets larger?

A

Bigger cells have less surface area relative to volume, so it becomes harder to get enough nutrients in and wastes out.

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19
Q

Why don’t cells grow very large?

A

As cells grow, volume increases faster than surface area, reducing diffusion efficiency and making nutrient intake and waste removal harder.

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20
Q

You take a dosage of milk of magnesia, which causes a high salt concentration in the fluids of your intestinal lumen (inside of intestines, where feces are formed). If increases or decreases in water in the intestinal lumen can affect the consistency of feces, then you would expect that:

a
Milk of magnesia likely helps cure diarrhea

b
Milk of magnesia likely helps cure constipation

c
Milk of magnesia would have no effect on feces consistency

A

B- Milk of magnesia likely helps cure constipation

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21
Q

Celery stalks that are immersed in freshwater for several hours become stiff. Similar stalks left in a 0.15 M salt solution become limp. From this, we can deduce that the freshwater:

a
and the salt solution are both hypertonic to the cells of the celery stalks

b
and the salt solution are both hypotonic to the cells of the celery stalks

c
is isotonic and the salt solution is hypertonic to the cells of the celery stalks

d
is hypertonic and the salt solution is hypotonic to the cells of the celery stalks

e
is hypotonic and the salt solution is hypertonic to the cells of the celery stalks

A

e
is hypotonic and the salt solution is hypertonic to the cells of the celery stalks

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22
Q

Just based on size, which do you think would lose/diffuse heat to the environment the fastest: my axolotl (<0.5 lb), my dog (~75 lbs), or me? (Think about the effects of surface area to volume ratio and size in your answer)
Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer.

a
Axolotl

b
Dog

c
Me

d
All would lose heat at the same rate

A

a
Axolotl

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23
Q

A normal cell has a low concentration of sodium on the inside and a high concentration on the outside. Yet, the net movement of sodium is out of the cell. Which of the following must be involved?

a
Osmosis

b
Facilitated diffusion

c
Active transport

d
Simple diffusion

e
Multiple of the above could be true

A

c
Active transport

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24
Q

What are two characteristics of Eukaryotes?

A

Includes vast majority of multicellular organisms
(e.g. plants, animals, fungi)
+ many single-celled
organisms (e.g. protists)

  • Very compartmentalized -
    many organelles,
    including the nucleus
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25
What are Organelles?
compartments with different functions in the cell
26
What are three characteristics of Prokayotes ?
Almost exclusively single- celled organisms (bacteria and archaea) * Contents usually free floating within cell (esp. DNA not in a nucleus) – rarely have many, if any, membrane-bound organelles * Various chemical differences – important for antibiotics!
27
Which two groups are prokaryokes sorted into?
Bacteria and Archaea (singular forms: bacterium and archaeon)
28
What are two characteristics of Archea?
Archaea: often in extreme environments – different membrane chemistry
29
What is the function of a plasma/cell membrane?
surrounds to cell and acts as a selectively permeable barrier
30
All _______ _______ have a plasma membrane
All living things have plasma membrane (bacteria, plants, animals, fungi, etc)
31
What is the function of the nucleus?
contains most of DNA in eukaryotic cells
32
What is the function of nucleoids?
Contains DNA of prokaryotic cells
33
What is the function of Ribosomes?
translate the message in RNA into proteins
34
Organelles allow cells that have them to compartmentalize different functions/activities → become more ______
efficient
35
Are prokaryotes or eukaryotes larger?
Prokaryotes also are much more numerous and much smaller (organelles allow eukaryotes to get bigger than prokaryotes)
36
What is the plasma membrane composed of?
Plasma membrane is composed of phospholipids, cholesterol, and various proteins, glycoproteins, and glycolipids
37
What happens when a specific protein is needed?
When a specific protein is needed, DNA is transcribed into RNA and the RNA is what leaves the nucleus
38
Where are ribosomes located?
Ribosomes may be free floating in cytoplasm or attached to rough ER
39
What is the endomembrane system involved in?
The endomembrane system is involved in the production, modification, and exportation of proteins and other macromolecules in eukaryotes
40
What organelles are part of the endomembrane system?
rough E R, smooth E R, and Golgi apparatus
41
What is the function of the Endoplasmic reticulum (ER)?
manufactures macromolecules for the cell
42
What does the Rough ER contain?
Rough E R – has ribosomes, parts of the surrounding membrane are continuous with the nuclear envelope
43
What is the function of the smooth ER and does it contain ribosomes?
Smooth E R – does not have ribosomes * Site of lipid manufacture/modification. has enzymes that detoxify some drugs and poisons
44
What is the function of Vesicles? (vesicle= vehicle= UPS)
small membrane-bound storage/shipping containers
45
What is the function of the Golgi apparatus?
rough products of the E R further processed, honed, and shipped to final destinations – e.g. outside the cell or to other organelles Protein folding, attach lipids, carbohydrates
46
What is the function of Lysosomes?
specialized vesicles for dissolving wastes, harmful chemicals/bacteria
47
What is the function of Peroxisomes?
Peroxisomes – variety of functions, redox reactions (is a chemical reaction where electrons are transferred between substances. Oxidation = losing electrons Reduction = gaining electrons)
48
What are the functions of the mitochondria?
1. convert stored energy (glucose and other macromolecules) into directly usable energy (A T P) 2. cellular respiration occurs here in eukaryotes 3. apoptosis, 4. signaling, 5. regulate cell Ca2+, 6. synthesis of some molecules
49
What is the function of Chloroplasts?
found in plants (and some single-celled eukaryotes); site of photosynthesis
50
What are the functions of a cell wall?
1. Imparts shape 2. Regulates cell volume 3. Prevents bursting of cells
51
What kind of organisms are cell walls found in?
Found in plants and bacteria plants: cellulose; bacteria: peptidoglycan
52
Does a cell wall take the place of a plasma/cell membrane?
cell walls DO NOT take place of cell membrane
53
What are the functions of Vacoles? (2)
1. Sometimes breaks down macromolecules (like lysosome) 2. Sometimes acts as storage of various molecules/ions
54
Which types of organisms contain vacoles?
found in plants and fungi – often quite large
55
Mitochondria have their own _____ and ______
Have their own DNA and ribosomes
56
What is endosymbiosis?
Mitochondria appear to have arisen from bacteria that were engulfed and subsequently incorporated into a eukaryotic cell, based on this and other evidence – this is called endosymbiosis
57
Mitochondria genes are usually inherited from ______
Are usually inherited from your mother – genetic mitochondrial diseases are passed down maternally because of this
58
Most cells produce material that forms a_______ outside of the _______
Most cells produce material that forms a layer outside of the plasma membrane
59
What is the extracellular layer outside of the plasma membrane composed of in eukaryotes?
fiber composites
60
What is the function of the fibers/filaments that compose the extracellular layer outside the plasma membrane in eukaryotes?
filaments/fibers that resist tension embedded in ground substance that resists compression
61
What is the extracellular layer outside of the plasma membrane called in animals?
extracellular matrix (ECM)
62
What is the cell wall in plants, bacteria, and other organisms composed of?
carbohydrates
63
The extracellular matrix is more ______ and contains substantially more ______ (especially collagen) than a cell wall does
The ECM is more flexible and contains substantially more protein (especially collagen) than a cell wall does
64
What are plasmodesmata?
Plant cells may have gaps in their cell walls and cell membrane called plasmodesmata
65
The ECM links up with and interacts with the_________ extracellular layer
Cytoskeleton
66
In multicellular organisms, adjacent cells connect and interact, largely through their _____
plasma membrane and/or the extracellular layer
67
What is the function of Nuclear pore complexes?
regulate traffic in/out of nucleus
68
How are larger molecules more selective in nuclear transport?
nuclear localization signals – essentially “zip codes” that mark for import
69
Why do different cells have different organelle proportions?
Because different cell types have different functions, they will have different proportions of the types of organelles. These proportions sometimes change over time (e.g. – more mitochondria in muscle cells after athletic training)
70
What is the nuclear envelope?
The double membrane that surrounds the nucleus
71
What is the function of nuclear pore complexes?
They allow transport of nucleotides and proteins into the nucleus and RNA/proteins out of the nucleus. DNA does not leave the nucleus.
72
Can small molecules pass through nuclear pores?
Yes, small molecules can passively diffuse through nuclear pores.
73
How do nuclear pores differ from typical transport proteins?
Nuclear pores are large protein complexes, allowing somewhat larger molecules to pass through, unlike smaller transport proteins.
74
What do larger molecules need to enter the nucleus?
They usually require a nuclear localization signal to be transported through the nuclear pore complex.
75
How does the endomembrane system ensure products reach the correct location?
It uses molecular “zip codes” to target products to the correct places.
76
What is an ER signal sequence?
The first several amino acids of some proteins that direct a free ribosome to bind to the ER and synthesize the protein inside the ER.
77
What happens to a ribosome after it finishes making a protein in the ER?
It detaches from the ER and becomes a free ribosome again.
78
How do proteins move from the ER to the Golgi apparatus?
They travel in vesicles that bud off from the ER.
79
What is the main function of the Golgi apparatus in protein transport?
It sorts proteins to their correct destinations using molecular tags that bind to receptors.
80
What is exocytosis?
When a vesicle merges with the plasma membrane, delivering proteins from inside the vesicle to outside the cell.
81
What happens to the vesicle membrane during exocytosis?
It becomes part of the plasma membrane, slightly enlarging it and delivering membrane proteins.
82
What is endocytosis?
When the plasma membrane pinches in to form a vesicle, bringing substances into the cell.
83
What is the cytoskeleto
an extensive system of fibers
84
What is the function of the cytoskeleton?
It provides shape and organization to the cell, but is also dynamic – movement of or within cell often involves parts of the cytoskeleton ex: . Provides paths for movement of vesicles within the cell, using motor proteins
85
What are gap junctions in animal cells?
Channels that connect the cytoplasm of adjacent cells, allowing molecules and ions to pass directly between them.
86
What is the plant equivalent of gap junctions?
Plasmodesmata, which also allow coordination of cellular activities between adjacent cells.
87
What are tight junctions?
Connections between adjacent animal cells that form a seal, preventing molecules from passing between cells.
88
What are desmosomes?
Structures that securely adhere adjacent cells using linking proteins.
89
How do desmosomes ensure selective cell adhesion?
The linking proteins in desmosomes only bind to cells with the same or complementary proteins.
90
What are two responses of cell to cell signaling?
Cell to cell signaling usually elicits one of two responses: 1) regulating protein synthesis (gene expression) or 2) activating proteins already present in the cell
91
How do signaling molecules like hormones act on cells?
They can reach almost every cell via the bloodstream but only affect target cells that have the appropriate receptor.
92
How can receptors for signaling molecules be regulated?
Receptors can change in number or sensitivity and can also be blocked.
93
What are the two main types of hormones?
Steroid hormones (lipid-based, from cholesterol) and nonsteroid hormones (protein or protein-like).
94
How do steroid hormones act on cells?
They are hydrophobic, cross the cell membrane, bind to receptors inside the cell, and usually affect gene expression.
95
How do nonsteroid (hydrophilic) hormones act on cells?
They bind to receptors on the cell surface, causing changes inside the cell through signal transduction.
96
What is signal transduction?
A process that changes the form of the signal inside the cell, often amplifying it through phosphorylation cascades.
97
How do phosphorylation cascades work?
Protein kinases add phosphate groups to activate or inactivate proteins, often starting with a second messenger.
98
How are signaling pathways turned off?
By phosphatases, which remove phosphate groups.
99
Can the same signaling molecule have different effects?
Yes, depending on the components of the signal transduction pathway in different cell types.
100
How do signaling pathways interact?
They often stimulate or inhibit other pathways, creating networked cellular responses.
101
How do unicellular organisms use signaling pathways?
For quorum sensing, detecting population size to coordinate group behavior.
102
What is signal transduction?
conversion of the form of a signal * Extracellular → intracellular Then activation of subsequent proteins
103
What are second messengers?
Second messengers: small molecules or ions that elicit intracellular responses * Second messenger often activate protein kinases
104
What are protein kinases?
enzymes that activate/inactivate other proteins by adding phosphate groups
105
What is energy?
Energy is the capacity to do work or supply heat
106
What is kinetic energy?
energy of motion – energy doing work
107
What is potential energy?
potential energy (energy of position – stored energy)
108
What are two important forms of potential energy in cells?
Chemical energy – energy stored in the chemical bonds of molecules (like carbohydrates and lipids). Nonpolar bonds store more energy than polar bonds. Electrochemical gradient energy – energy stored due to differences in charge and concentration across a membrane.
109
What is the 1st law of thermodynamics?
Energy cannot be created or destroyed, but can be changed between form
110
What is the 2nd law of thermodynamics?
Entropy (“disorder”) never decreases – in a spontaneous reaction, it will increase in the system and/or its environment
111
Why are energy transformations never completely efficient in biological systems?
Because some energy is always lost to the environment as heat.
112
What is a spontaneous reaction?
A reaction that occurs without a net input of energy. It is most likely when: Products have less potential energy, and/or Products have more entropy than reactants.
113
What is the ΔG of spontaneous (exergonic) reactions?
Spontaneous (exergonic) reactions have negative ΔG.
114
What is the ΔG of nonspontaneous (endergonic) reactions?
Nonspontaneous (endergonic) reactions have positive ΔG.
115
What does ΔG depend on?
ΔG depends on: Change in enthalpy (ΔH) Change in entropy (ΔS) Temperature (T)
116
How do cells drive endergonic reactions?
By coupling them to exergonic reactions. Often done by: Phosphorylation (adding phosphate groups) Redox reactions (adding/removing electrons) These increase the potential energy of a reactant.
117
What must happen during a chemical reaction?
Bonds must be broken and formed Reactants must collide in the correct orientation
118
How does temperature or reactant concentration affect uncatalyzed reactions?
Increasing temperature or concentration → More collisions → Faster reaction rate
119
What is activation energy?
The initial energy required to reach the transition state (where bonds are breaking and forming). Even exergonic reactions require activation energy.
120
How do enzymes speed up reactions?
Bring substrates together Stabilize the transition state Lower activation energy They do NOT change ΔG.
121
Does an enzyme change during a reaction?
Yes. It usually changes shape temporarily during the reaction.
122
What are cofactors, coenzymes, and prosthetic groups?
Non-protein helpers required for enzyme function. They assist at or near the active site.
123
How does temperature affect enzyme activity?
Rate increases with temperature Up to an optimal point Then decreases if enzyme denatures
124
How does pH affect enzymes?
Changes in pH can disrupt 3D structure → Alters or destroys function.
125
How does substrate concentration affect reaction rate?
Rate increases as substrate increases BUT eventually plateaus when enzymes become saturated (maximum rate reached).
126
What is competitive inhibition?
A molecule binds to the active site, blocking the substrate.
127
What is allosteric regulation?
A molecule binds away from the active site, changing enzyme shape → activates or inhibits enzyme.
128
How can phosphorylation regulate enzymes?
Adding a phosphate group can change enzyme shape → Activate or inactivate the enzyme.
129
How are metabolic pathways often regulated?
By feedback inhibition — A later product inhibits an earlier enzyme in the pathway.
130
What is a pathogen?
An infectious agent that causes disease. Includes (smallest → largest): Prions Viruses Bacteria Protists Fungi Parasitic worms
131
Why are viruses often considered non-living?
They cannot reproduce or carry out metabolism without a host cell. They must hijack a host cell’s machinery.
132
What are viruses made of?
Protein coat DNA or RNA (single or double stranded) Some have lipid/carbohydrate envelopes (from host membrane).
133
How do enveloped viruses get their lipid membrane?
They bud from the host cell membrane instead of bursting out.
134
What is a retrovirus?
A virus that: Uses reverse transcriptase Converts RNA → DNA Inserts DNA into host genome Sometimes permanently.
135
Why is reverse transcriptase important in biotechnology?
It allows scientists to convert RNA into DNA (used in research and genetic engineering).
136
Why are viruses host-specific?
Specific viral surface proteins match specific host cell receptors. Example: bacteriophages infect specific bacteria.
137
Are most bacteria harmful?
No. Most are harmless or beneficial and form our microbiota.
138
How do bacteria reproduce?
Asexually (binary fission) → very rapid reproduction.
139
How do bacteria gain genetic diversity if they reproduce asexually?
Exchange plasmids Pick up DNA from environment DNA transfer via viruses
140
What are plasmids?
Small circular DNA separate from the main chromosome. Often carry genes useful in special conditions (e.g., antibiotic resistance).
141
How do pathogenic bacteria cause disease?
By releasing destructive enzymes or toxins.
142
How do antibiotics work?
They target bacterial structures human cells don’t have, such as: Cell walls Bacterial ribosomes DNA not enclosed in a nucleus
143
Why is antibiotic resistance increasing?
Overuse in agriculture Using antibiotics for viral infections Not finishing full antibiotic courses
144
What is cellular reparation?
Cellular respiration allows organisms to convert energy stored in molecules with high potential energy (such as glucose) to energy stored in the bonds of ATP
145
What are the 4 stages of cellular respiration (when oxygen is available)?
In eukaryotes and many prokaryotes, it consists of four stages: glycolysis, pyruvate processing, citric acid cycle, and electron transport chain/oxidative phosphorylation (when oxygen is available)
146
What is the purpose of cellular respiration?
To convert energy stored in high-potential molecules (like glucose) into energy stored in ATP.
147
What happens during the first three stages of cellular respiration?
Energy from glucose is transferred to: NADH FADH₂ All CO₂ is released by the end of the citric acid cycle. 4 ATP are produced by substrate-level phosphorylation total.
148
Where does glycolysis occur?
In the cytosol of both eukaryotes and prokaryotes.
149
What are the products of glycolysis (per glucose)?
2 pyruvate 2 net ATP (4 made, 2 used) 2 NADH
150
Why is glycolysis considered an energy investment and payoff process?
It uses 2 ATP early on but produces 4 ATP later → net gain of 2 ATP.
151
How is glycolysis regulated?
At the phosphofructokinase (PFK) step. ATP is both a substrate and an allosteric inhibitor. This is the first irreversible step.
152
Where does pyruvate processing occur?
Mitochondrial matrix (eukaryotes) Cytosol (prokaryotes)
153
What are the products of pyruvate processing (per glucose)?
2 CO₂ 2 NADH 2 Acetyl CoA
154
What enters the citric acid cycle?
The acetyl group from acetyl CoA.
155
What are the products of the citric acid cycle (per glucose)?
6 NADH 2 FADH₂ 2 ATP (or GTP) 4 CO₂
156
What is the role of the electron transport chain?
To transfer electrons from NADH and FADH₂ to O₂ through redox reactions.
157
What is the final electron acceptor in aerobic respiration?
Oxygen (O₂), which forms water.
158
How does the electron transport chain generate ATP?
Electron energy pumps H⁺ across the inner mitochondrial membrane, creating a proton gradient. ATP synthase uses this gradient to make ATP.
159
About how much ATP is produced by oxidative phosphorylation per glucose?
About 25 ATP
160
When does fermentation occur?
When oxygen or the electron transport chain is unavailable.
161
How much ATP does fermentation produce per glucose?
2 ATP (from glycolysis only).
162
What is the purpose of fermentation?
To regenerate NAD⁺ so glycolysis can continue.
163
Difference between lactic acid and alcoholic fermentation?
Lactic: pyruvate → lactate Alcoholic: pyruvate → CO₂ + ethanol
164
How do some bacteria/archaea perform respiration without oxygen?
They use alternative final electron acceptors in their electron transport chain. They produce more ATP than fermentation but less than aerobic respiration.
165
How do fats enter cellular respiration?
Glycerol enters glycolysis Fatty acids are broken into 2-carbon units → acetyl CoA
166
How do proteins enter cellular respiration?
Amino acids lose their amino group and enter at various points depending on their structure.
167
How can cellular respiration intermediates be used in anabolic pathways?
They serve as building blocks for all four macromolecules.
168
What causes reactive oxygen species (ROS) to form?
When oxygen accepts electrons prematurely in the electron transport chain, often when the proton gradient is very high and the chain slows down.