Exam 2, Lecture 4

Question 1

List 4 types of fungal stains.

Answer 1

• Lactophenol cotton blue
• GMS (Gomori methenamine silver)
• PAS (Periodic acid-Schiff)
• Mucicarmine (mucin)

Question 2

List 4 types of parasite stains.

Answer 2

• Wright’s Giemsa
• Wet Mounts
• Trichrome
• Modified acid fast

Question 3

What organisms can you identify w/Lactophenol cotton blue stain?

Answer 3

Fungi:

- Histoplasma

Question 4

What organisms can you identify w/Wright’s Giemsa stain?

Answer 4

Parasites:

• Histoplasma
• Leishmania
• Trypanosomas
• Malaria

Question 5

What organisms can you identify w/GMS stain?

Answer 5

Fungi:

• Yeast cells and hyphae
• *Pneumocystis carinii

Question 6

What organisms can you identify w/mucicarmine (mucus) stain?

Answer 6

Fungi:

- Cryptococcus *capsule

Question 7

What organisms can you identify w/PAS stain?

Answer 7

Fungi:

- Yeast cells and hyphae

Question 8

What organisms can you identify w/wet mounts?

Answer 8

Parasites:

- Giardia

Question 9

What organisms can you identify w/trichrome stain?

Answer 9

Parasites:

- Giardia

Question 10

What organisms can you identify w/modified acid-fast stain?

Answer 10

• Parasites

- Nocardia (a bacteria)

Question 11

Acid-fast stain stains what 4 pathogens?

Answer 11

• Mycobacteria
• Nocardia
• Cryptosporidia
• Cyclospora