Bacteriology > Exam 3: E. coli > Flashcards

Exam 3: E. coli Flashcards

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1
Q

Where are Enterobacteriae commonly found

A

Water, soil but mostly GIT

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2
Q

What is the purpose of Enterobacteria in the GIT

A
  1. Absorption of food
  2. Waste processing
  3. Vit. K production
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3
Q

What endotoxin is produced by Enterobacteria that causes endotoxic shock?

A

Lipid A

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4
Q

Are Enterobacteria gram + or -

A

Gram -

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5
Q

What are the 3 coliform bacteria?

A
  1. E. coli
  2. Klebsiella
  3. Enterobacter
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6
Q

What are the 5 Enterobacterium we learned about

A
  1. Escherichia
  2. Salmonella
  3. Klebsiella
  4. Proteus
  5. Yersinia
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7
Q

What are the two strains of E. coli?

A
  1. Intestinal strains

2. Extra-intestinal Strains

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8
Q

What are some things that extra intestinal E. coli cause?

A
  1. Resp
  2. Septicaemia
  3. UTI
  4. Mastitis
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9
Q

What are the two categories of E. coli virulence factors?

A
  1. Cell associated

2. Extracellular

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10
Q

What are the 4 cell associated virulence factors of E. coli?

A
  1. Endotoxin
  2. Capsule
  3. Fimbrial
  4. Non-fimbrial adhesins
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11
Q

What are the 3 Extracellular virulence factors of E. coli

A
  1. Enterotoxins
  2. Cytotoxins
  3. Siderophores
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12
Q

What are the 5 Enteric pathotypes of E. coli

A
  1. Enterotoxigenic (ETEC)
  2. Enterohemorrhagic (EHEC)
  3. Verotoxigenic (VTEC)
  4. Enteropathogenic (EPEC)
  5. Rabbit pathogenic (RPEC)
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13
Q

What are the 3 Extra-intestinal pathotypes of E. coli

A
  1. Avian pathogenic (APEC)
  2. Necrotoxigenic (NTEC)
  3. Mastitis causing E. coli
  4. MMA in pigs
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14
Q

What are the 6 pathotypes of E. coli that affect pigs

A
  1. ETEC
  2. VTEC
  3. EPEC
  4. UTI
  5. MMA
  6. Septicaemiae
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15
Q

Which Pathotype of E. coli causes watery D+ in piglets aged 2-4 weeks

A

ETEC

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16
Q

Why are adhesion factors in ETEC important?

A

Used for vacc.

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17
Q

Where are the receptors for the Adhesion factors of ETC in pigs?

A

Small intestine

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18
Q

What are the 6 adhesion factors of ETEC?

A 
F4
F5
F6
F18
F41
Non-fimbral adhesions
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19
Q

What are the two types of Enterotoxins on the plasmids of ETEC?

A
  1. Heat labile

2. Heat stable

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20
Q

T/F. There is an equilibrium b/t maternal immunity and infection pressure in ETEC?

A

T

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21
Q

What are the 3 things that cause a disturbance in equilibrium leading to dz in ETEC?

A
  1. Inc. infection pressure
  2. Dec Maternal immunity
  3. Appearance of new type
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22
Q

What are the C/S of ETEC?

A
  1. dehydration
  2. Smelly D+
  3. inc. thirst
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23
Q

How do you Dx ETEC in pigs

A
  1. Agg. test
  2. PCR
  3. Detection of Virulence factors
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24
Q

How do you tx. ETEC in pigs?

A

Fluids and antimicrobials

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25
T/F. There are vacc. available for ETEC
T
26
What strain of E. coli causes Edema Dz in pigs
VTEC
27
Where is Adhesion of VTEC in pigs
Small intestine
28
What are the 3 virulence factors of VTEC in pigs
1. Adhesion 2. Exotoxin 3. Endotoxin
29
What Exotoxin is produced by VTEC in pigs?
VT2E/Stx2e
30
What do endotoxins produced by VTEC cause in pigs?
acute death
31
What Adhesion factor is important in VTEC infections in pigs
F18
32
What are the C/S of VTEC in piglets
1. acute mortality 2. D+ 3. Anorexia 4. Edema 5. Swollen eyelids
33
How do you Dx VTEC in pigs
1. C/S 2. Pathology 3. Bacteriology 4. PCR
34
how do you Tx VTEC in pigs?
Fast and ABx
35
What pathotype of E. coli causes D+ at weaning
EPEC
36
What is used in EPEC infection in piglets for initial attachement
BFP bundle forming pili
37
How do you Dx EPEC in pigs
Isolation and PCR
38
What is the most important pathogen causing UTI in pigs
E. coli
39
DDx of UTI in pigs
Actinobaculum suis
40
Where does E. coli causing UTI in pigs come from?
Intestine
41
Predisposing factors For E. coli UTI in pigs
1. Hygiend 2. Water intake 3. Obstipation 4. Age
42
Symptoms of E. coli UTI in pigs
1. Anorexia 2. Hematuria 3. Vag. exudate 4. General malaise
43
how do you Dx E. coli UTI in pigs
Bacteriology
44
Tx of UTI in pigs
Culling | Abx
45
Disease in pigs secondary to enteric infection
Septicaemiae
46
What are the 3 strains of E. coli that can infect cows
1. ETEC 2. EPEC 3. EHEC 4. NTEC (septicemic)
47
ETEC infects cows what age?
< 3 days old
48
C/S of ETEC in calves
1. Watery D+ | 2. Dehydration
49
What are the 2 virulence factors of ETEC in cows
1. Fimbrae | 2. Toxins
50
What are the fimbriae associated with ETEC in cows
F5 F 41 F17 CS31A
51
What are the two toxins associated with ETEC in calves
Sta and Stb
52
T/F. All ETEC toxins in cattle are heat labile
F. Heat stable only
53
how do you Dx ETEC in catle
Elisa | Culture and AGG. or PCR
54
Which strain of E.coli in cattle is zoonotic?
EHEC
55
Cows 1-8 wks with bloody D+ could be infcted with what 2 strains of E. coli
EHEC and EPEC
56
Where is EHEC and EPEC in cattle found
Lg. intestine
57
Disease in bovine caused by E. coli
Mastitis
58
What are the virulence factors of E. coli Mastitis in cows
None
59
What is the Strain of E. coli that causes Septicaemia in cows
NTEC
60
What are The virulence factors of NTEC which causes septicaemia in cows
1. Serum resistance 2. Fe uptake 3. Endotoxin 4. Capsule 5. Adhesion factors 6. DNF1 and 2 toxins
61
How do you Dx septicaemic E. coli in bovines
C/S | Isolation and PCR
62
How do you tx Septicaemic E. coli in bovines
1. NSAIDS 2. Antibiotics 3. Gamma globulins
63
How do you prevent Septicemic E. coli in cattles
Colostrum
64
Where is E. coli isolated in dogs and cats
Poops
65
What are the two Diseases caused by E. coli infection in dogs
1. Cystitis | 2. Pyometra
66
E. Coli strain in birds
APEC
67
What is the most important bacterial Dz of broilers and layers
APEC
68
What are the specific serotypes of E. coli in poultry
01 02 078
69
T/F. GI signs are the most important C/S in broilers infected with E. coli
F: APEC is Extra enteric, so no GI signs
70
What are the C/S of E. coli infection in birds
1. Septicemia 2. Resp 3. Peritonitis 4. Scabby hip
71
What is the most common C/S of E. coli infection in layers
Peritonitis
72
Dx of E. coli in birds
1. C/S | 2. Isolation
73
What are the 4 DDx of E.coli in birds
1. Yolk rest infection 2. Acute sepsin 3. Coligranuloma 4. Airsacculitis
74
How do you Tx E. coli in birdss
Difficult, so do AST
75
How do you prevent E. coli in birds
1. hygiene 2. Stress 3. Temp. 4. Vacc
76
What 2 serotypes cause Scabby hip in broilers?
078 and 02
77
how do you prevent scabby hip?
Dec. number of animals housed together
78
How do you Dx E. coli in rabits
1. C/S | 2. Isolation and subtyping
79
T/F. Isolation and subtyping is not necessisary in rabbit E. coli infections
F. Necessary! some types are more lethal
80
How do you tx E. coli in rabbits
Abx
Bacteriology
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