Method for Creatine Kinase-Mb Determination
Immuno-inhibition method by Wurzburg
Creatine kinase are dimeric molecules composed of:
M & B subunits
isoenzymes used in CK
MM
MB
BB
M & B subunits are?
immunologically distinct
CK-MM & CK-MB are distributed primarily in the _________ & ________?
Skeletal Muscle & Heart Muscle
CK-BB is present mainly in the?
brain
tissue composed of smooth muscle
Isoenzymes are separated via:
Electrophoresis
column anion exchange
batch anion exchange chromatography
Isoenzymes that are completely precipitated by homologous rabbit antisera
MM
BB
Linearity of CK-MB
1500 IU/L
Factor
3376