Solute
Substance dissolved in a solution
Solvent
The liquid that dissolves the solute to form a solution
Solution
Mixture of dissolved solute in a solvent
Osmosis
Movement of water from low solute concentration to high solute concentration to achieve equilibrium
Tonicity
The ability of a solution to make water move into or out of a cell by osmosis
Hypotonic
Isotonic
Equal solute concentration
RBCs are in an isotonic solution
Hypertonic
Solution has a higher solute concentration
RBCs are in a hypertonic solution
Why is 0.15 M NaCl isotonic with the 0.3M RBC cytoplasm?
NaCl dissociates into two parts: Na+ is 0.15 M and Cl- is 0.15 M so when you add them together, it equals 0.3 M and is equal to the RBC cytoplasm
Can NaCl cross the cell membrane? Why?
No, NaCl is charged and needs a protein transporter to cross the membrane, something that is not available in the RBCs
Why does 0.3 M Ethylene glycol cause lysis even though the solution is isotonic?
E. glycol wants to achieve equilibrium itself and over time will enter the cell
Opaque solution is either
Isotonic of hypertonic (RBCs remained intact)
0.15 M or 0.8 NaCl
More transparent solution is
Hypotonic
Water or 0.3 M Ethylene Glycol
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