How does the genetic code in a gene code for tubulin
- 3 bases code for one specific amino acid
- Sequences of bases/ triplets determine the sequence of amino acids
Describe three cellular functions of the cytoskeleton
- enables organelles to move through vesicles in the cytoplasm
- can change shape of plasma membrane endo/exocytosis
- microtubules are involved in the formation of cilia that waft mucous in the airways
Suggest two ways tubulin is essential for protein synthesis/ secretion
- movement of mRNA from nucleus to ribosome
- movement of polypeptides through RER
Explain how to measure the diameter of the nucleus of a WBC, when observing the cells through a light microscope
1) Use eyepiece graticule
2) Calibrate graticule using stage micro meter
3) Measure the diameter of the nucleus in graticule units
4) Take repeat measurements and calculate a mean diameter
5) Use calibrated eyepiece units to calculate the diameter of the nucleus
Two obvs that a cell is in G2
- Large number of organelles
- more DNA
- no visible chromosomes
- nuclear membrane present
DNA precipitation practical
1) Mix culture of WBC with detergent
2)Add salt
3) Add enzyme
4) Place in water bath at 40 degrees
5) Filter culture
6) Gently pour ice cold onto the precipitate
In what scenario would the cells not need to be crushed in DNA precipitation
When the cells don’t have a cell wall
What enzyme is used in DNA precipitation
Protease - breaks down histone proteins associated with DNA
Q10
rate of reaction (t +10 degrees) / Rate of reaction at T
What makes glucose easily transported in animals
Polar
