What is capacitation?
Final maturation of sperm inside the female reproductive tract
How is the glycoprotein coat of sperm removed?
In the uterus removed by protease enzymes
What is caused by the removal of the glycoprotein cap?
Membrane becomes more permeable to calcium increasing cAMP causing strong whip like tail movement
What is digested by the acrosomal contents?
Digest the zone pellucida
What is caused by the calcium waves created as the sperm binds with the egg?
Cortical granules are released making the egg impenetrable by other sperm and meiosis of the oocyte resumes
What are the common reasons assisted fertilisation is used?
Blocked oviducts, blocked vas deferens and female age
What are the stages of assisted fertilisation?
Superovulation, oocyte harvesting, sperm harvesting, capacitation of sperm, mixing of sperm and oocytes, observation of early development and embryo transfer
What must be extracted with the oocyte during assisted fertilisation?
Granulosa cells
What is meant by cleavage when referring to early growth?
Mitosis with no growth
What occurs at the 4 cell stage?
Embryo starts making its own mRNA allowing its own genome to become active
What do the outer cells become?
Trophoblast which forms the placenta
What do the inner cells become?
Embryo proper becoming the inner cell mass
What must the embryo do before implanting into the uterine wall?
Hatch from the zone pellucida
What happens as embryo impacts into the uterus?
Trophoblast starts to grow into the uterus