1
Q
What is the principle of total and direct bilirubin?
A
Diazo reaction of ehrlich
2
Q
Mechanism of total and direct bilirubin
A
(Sulfanilic acid+HCl) + (sodium nitrite)= (nitrous acid)
(Nitrous acid) reacts to form (diazotized sulfanilic acid) + (bilirubin) = (azobilirubin)
3
Q
total bilirubin reagent
A
sulfunilic acid diluted with hcl. stabilized + accelerator
4
Q
bilirubin oxidant
A
sodium nitrite
5
Q
bilirubin calibrator
A
n-1-naphthylethylenediamine dihydrochloride
6
Q
direct bilirubin reagent
A
sulfanilic acid diluted with hcl. stabilizer addded
7
Q
sample of choice for total and direct bilirubin
A
fasting serum/plasma spx
8
Q
interfering substances of T&D bilirubin
A
gross hemolysis and lipemic serum
CCHM311
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