Ka,kb always been fixed
اللي بيتغير تركيز الحمض وتراكيز القاعدة المرافقة والhبلس للحمض ونفس الاشي للقاعدة
Buffer capacity
Depends on the ratio A-/HA
Inflection point
Happened in the titration reaction when the ph=pka
Equivalent point
All the acid is dissociated at this point
Plateau region
Buffer region
Titration curve
Relationship between the ph and the OH- equivalent
Indicator to detect the equivalent point
Phenolphathalien is colorless in acidic, pink in basic
Physiological buffer
1-
2-
3-
What is the staining technique that i can use to visualize the proteins in SDS page
1- coomassie blue
2- radioactivity
3-western blottin