Forward Genetics

Question 1

Give the definition of forward genetics.

Answer 1

Seeks to find the genes encoded by DNA that are responsible for a phenotype of interest.

Question 2

Give the classical genetics strategy.

Answer 2

1. isolate mutants with a phenotype of interest
   - choose a good organism
   - find a mutant of that organism – can generate mutations by applying mutagens
   - screen for mutants with desired phenotype
2. define genes responsible for these phenotypes
3. clone/identify the genes
4. analyse the genes to predict encoded proteins

Question 3

Describe the process of cloning genes by mutant rescue.

Answer 3

• transform cells with gene/cDNA library

- isolate transformants which rescue, or restore wild-type phenotype