FRET

Question 1

What does FRET stand for?

Answer 1

Forster resonance energy transfer

Question 2

How does a donor fluorophore transfer energy to the acceptor?

Answer 2

Through non-radiative dipole-dipole coupling

Question 3

What is the efficiency inversely proportional to?

Answer 3

The sixth power of the distance between the donor and the acceptor

Question 4

Describe fluorescence quenching?

Answer 4

There is FRET between a fluorescent probe and a quencher

Question 5

What quencher can be used?

Answer 5

TaqMan

Question 6

What does TaqMan do?

Answer 6

As it extends the primer and bumps into the probe the probe will degrade and release the fluorophore