gene mapping

Question 1

What two key pieces of information does a gene map show?

related and far

Answer 1

it shows the relative order of genes on a chromsome and the distance between them

Question 2

what term describes the conservation of gene order on chromosomes across different species

artifical

Answer 2

conserved or shared synteny

Question 3

What is the process of identifying genes linked to diseases or traits using gene maps called?

purely based on chromsomal location

Answer 3

positional cloning

Question 4

in humans, one centiMorgan (cM) is approximately equivalent to what physical distance?

Answer 4

one megabase (Mb), which is one million base pairs

Question 5

what are the three main types of gene maps discussed?

Answer 5

genetic/linkage
cytogenetic
physical

Question 6

the ultimate physical map of the human chromosomes, their DNA sequence, was provided by which project

Answer 6

Human Genome Project

Question 7

what mehod was used to create the first physical maps?

Answer 7

using restriction enzymes that cut DNA at specific sites, creating a restriction enzyme map

Question 8

on a cytogenetic map, what name is given to the short arm of a chromosome?

french

Answer 8

the p arm (from petit)

Question 9

on a cytogenetic map, what name is given to the long arm of a chromosome?

Answer 9

the q arm

Question 10

what staining technique produces a characteristic pattern of light and dark bands on chromsomes for cytogenetic mapping?

charli damelo friend

Answer 10

giemsa staining
after mild proteolytic digestion
(G-banding)

Question 11

the gene mutated in cystic fibrosis, CFTR, is located at what specific position on a human cytogenetic map?

Answer 11

7q31.2

Question 12

in the cytogenetic location 7q31.2, what does the 7 represent

Answer 12

chromosome 7

Question 13

in the cytogenetic location 7q31.2, what does the q represent

Answer 13

The long arm of the chromsome

Question 14

what is the term for genes located on the same chromsome that tend to be inherited together

Answer 14

linked genes (or showing linkage)

Question 15

genes on the same chromosome may violate which of Mendel’s laws?

Answer 15

Mendel’s second law of independent assortment

Question 16

during which phase of meiosis can crossing over occur, leading to the separation of linked alleles?

Answer 16

prophase i

Question 17

the frequency of crossing-over between two gene loci is proportional to what

do they lik each other

Answer 17

the physical distance between them on the chromosome

Question 18

in the absence of crossing over between two linked genes, what type of gametes are produced

adults only

Answer 18

only parental gametes

Question 19

what is the genetic consequence of a single cross-over event between two linked gene loci?

both adult and babies

Answer 19

the production of both parental and recombinant gametes

Question 20

a higher frequency of crossing over between two genes leads to a higher frequency of which type of gametes?

yk its the one

Answer 20

recombinant gametes

Question 21

what is the maximum percentage of recombinant gametes produced by independent assortment?

Answer 21

50%

Question 22

what does a recombination frequency of less than 50% between two genes indicate?

they locked in

Answer 22

the genes are linked on the same chromosome

Question 23

a recombination frequency of 50% indicates that genes are either on different chromosomes or ______.

they hate each other

Answer 23

are very far apart on the same chromosome

Question 24

what is the formula for calculating recombination frequency (RF)

Answer 24

RF = (no. of recombinant progeny / total no. of progeny) x 100

Question 25

what unit is used to measure genetic distance on a linkage map

Answer 25

centiMorgans (cM)

Question 26

a recombination frequency of 1% is equivalent to what genetic distance?

Answer 26

1 centiMorgan (cM)

Question 27

a genetic distance of 10.7 cM between two genes corresponds to what recombination frequency

Answer 27

10.7%