genetic technology

Question 1

what are the steps for transfer of a genes into another organism?

Answer 1

1. desired gene is identified
2. desired gene is isolated for it to be extracted
3. the gene is multiplied using polymerase chain reaction
4. the gene is inserted into a vector
5. the vector delivers the gene to the cells of the organism
6. cells expressing the new gene are identified (using gene markers) and then cloned

Question 2

What are three ways to extract the isolated desired gene?

Answer 2

• it can extracted by cutting it from a chromosome using enzyme restriction endonucleases
• the enzyme reverse transcriptase can also be used to make a single strand of complementary DNA (cDNA) from mRNA
• the gene can also be created artificially using nucleotides

Question 3

what is meant by a recombinant DNA?

Answer 3

DNA made by joining pieces from two or more different sources

Question 4

What is meant by a transgenic organism?

Answer 4

organism that expresses the genes that have been attained from another organism because of rDNA (recombinant)