Glucose Methodologies (Specimen consideration and Handling)

Question 1

Standard specimen

Answer 1

Serum

Question 2

5% lower compared to serum

Answer 2

Plasma

Question 3

10-15% lower than serum

Answer 3

Whole blood

Question 4

Serum, plasma, whole blood must not be collected in the arm with IV fluid contains 5% dextrose because 10% contamination in blood caused by 5% dextrose can increase plasma glucose by

Answer 4

500 mg/dL (falsely elevated)

Question 5

FBS is 7mg/dL higher than serum
OGTT: 20-70 mg/dL or 20-25% higher than venous blood

Answer 5

Capillary

Question 6

Normal range of glucose in CSF

Answer 6

60-70% plasma glucose

Question 7

Is an enzyme that is part of glycolysis

Answer 7

Enolase

Question 8

Can inhibit glycolysis by inhibiting the enzyme
enolase by forming complex with magnesium (Mg2+)

Answer 8

Sodium Fluoride

Question 9

Can cause 7mg/dL/hr decrease

Answer 9

Room temp (20-25degC)