Haemophilus

Question 1

Haemophilus are normal flora of

Answer 1

respiratory

Question 2

Haemophilus optimal growth

Answer 2

35-37C, 5% CO2

Question 3

Haemophilus Gram stain

Answer 3

Gram Negative coccobacilli, pleomorphic

Question 4

H. influenzae Type B is surrounded by

Answer 4

polysaccharide capsule

Question 4

H. influenzae Type B is covered by

Answer 4

antiphagocytic polysaccharide capsule

Question 5

H. influenzae Type B is spread through

Answer 5

respiratory droplets

Question 6

H. influenzae causes which diseases?

Answer 6

1. Pneumonia
2. Cellulitis
3. Sepsis
4. Meningitis

Question 7

H. influenzae Type B virulence factors

Answer 7

1. Capsule
2. IgA protease
3. Lipid A
4. Pili

Question 8

H. ducreyi disease

Answer 8

chancroid STI

Question 9

H. ducreyi Gram stain morph

Answer 9

GNR school of fish/railroad tracks

Question 10

H. ducreyi isolation

Answer 10

1. CHOC w/ vancomycin and growth factors
2. 33C 7-10 days
3. CO2, high humidity

Question 11

H. aegyptius infections

Answer 11

purulent conjunctivitis
Brazilian Purpuric Fever

Question 12

Haemophilus ID tests

Answer 12

1. Latex Agg
2. Hemolysis
3. X & V factor
4. porphyrin test

Question 13

X Factor

Answer 13

Hemin, heat stable

Question 14

V Factor

Answer 14

Nicotinamide Adenine Dinucleotide (NAD), heat labile

Question 15

Beta hemolytic Haemophilus

Answer 15

H. haemolyticus & H. parahaemolyticus

Question 16

Gamma hemolytic

Answer 16

H. influenzae & H. parainfluenzae

Question 17

H. ducreyi factor requirement

Answer 17

X Factor (hemin)

Question 18

H. parainfluenza factor requirement

Answer 18

V Factor (NAD)

Question 19

H. parahaemolyticus factor requirement

Answer 19

V Factor (NAD)

Question 20

H. influenzae factor requirement

Answer 20

XV Factor

Question 21

H. haemolyticus factor requirement

Answer 21

XV Factor

Question 22

Released by S. aureus hemolysis allowing satellite colonies

Answer 22

X Factor (hemin)

Question 23

produced by S. aureus allowing satellite colonies

Answer 23

V Factor (NAD)

Question 24

Porphyrin test conversions

Answer 24

1. ALA 2. Porphobilinogen 3. Porphyrins 4. Hemin

Question 25

ALA positive

Answer 25

H. parainfluenzae & H. parahaemolyticus (red/orange)

Question 26

ALA negative

Answer 26

H. influenzae & H. haemolyticus