Hard questions part 2

Question 1

Oestrogen is lipid soluble. Which molecule does oestrogen interact with when it changes cell activity

Answer 1

DNA

Question 2

How can something lead to excessive production of a hormone

Answer 2

1. Cells produce more hormone than normal
2. Due to up regulation of genes
3. Synthesising more hormone/proteins
4. Other tissues could be stimulated to produce more hormone than normal

Question 3

Suggest three improvements to this method that would increase the validity of the slides produced

1. Select a blade
2. Cut Heliamphora tissue
3. Select best pieces
4. Place on slide
5. Add cover slip

Answer 3

1. sharp blade (should be selected) so slide is thin enough that individual cells are visible / resolution is high
2. Use a microtome for slicing pieces of tissue (thinly) so slide is thin enough , individual cells are visible /resolution is high
3. Select thin(nest) slides to ensure maximum light can penetrate sample
4. Wet mount- prevents dehydration / distortion of tissue
5. Squash slide- easier to see individual cells / allows light to penetrate tissue more easily