First missed menstrual period:
100, 000 mIU/mL
Principle of PT:
Rapid chromatographic immunoassay
Detected in urine/serum/plasma:
7 - 10 days after conception
Sensitivity:
25 mIU/mL
[2] Control line is composed of:
- Good polyclonal antibodies
- Colloidal Gold Particles
Addition of __ drops ( 50 uL). Specimen migrates via capillary along the membrane to react with the colored conjugate.
2 drops
Positive reaction: HCG react with the specific ___________ to form a line at the test region of the membrane.
Anti - HCG specific antibody - colored conjugate
Specimen of choice:
First morning urine or serum
Contains the highest conc of HCG:
First morning urine
[3] Samples with visible precipitate:
- centrifuge
- filter
- allow to settle
48 hours
[storage]
2 - 8 C
Immediate
[storage]
Room temp
Months
[storage]
- 20 C
[2] False positive:
- Trophoblastic disease
- Non - trophoblastic neoplasm
[4] Non - trophoblastic neoplasm
- Prostate cancer
- Testicular cancer
- Breast cancer
- Lung cancer
Must be confirmed with other tests.
Ultrasound
Two distinct red lines appear. One line should be in the control region (C) and another line should be in the test region (T).
[test reporting]
Positive
One red line appears in the control region (C). No apparent red or pink line appears in the test region (T).
Negative
Control line fails to appear.
Invalid
PEAK (1st trimester):
100, 000 - 200, 000 mIU/mL
Where is HCG produced shortly after fertilization?
Developinh placenta
How soon after conception can HCG typically be detected in urine, serum, or plasma?
7 - 10 days
What can cause a false negative result due to high dilution?
Low specific gravity
