Hematology

Question 1

Which part of the blood smear should be used to look for platelet clumps?

Answer 1

feathered edge

Question 2

What magnification should you use to look for platelet clumps?

Answer 2

4x or 10x

Question 3

Which part of the bloodsmear should you use to count platelets for estimation?

Answer 3

monolayer

Question 4

What magnification should you use to count platelets for estimation?

Answer 4

100x oil

Question 5

Platelet clumping will do what to the automated count and estimate?

Answer 5

falsely decrease it

Question 6

What is the equation for platelet estimation?

Answer 6

average number of platelets per oil field x 20,000

Question 7

How many fields should you count platelets in for a per field average?

Answer 7

5-10

Question 8

What should you use to label slides?

Answer 8

pencil or wax pencil

Question 9

Why should we not use sharpies to label slides?

Answer 9

because it will dissolve in fixative

Question 10

What is the minimum information you should label a slide with?

Answer 10

patient name or ID and date

Question 11

Which cells are segmented?

Answer 11

• neutrophils
• eosinophils
• basophils

Question 12

Which cells are non-segmented?

Answer 12

• monocytes
• lymphocytes

Question 13

The correct sequence for staining blood smears in Diff-Quik type stains is what?

Answer 13

Light blue (5 x 1 sec dips),
Red ( 5 x 1 sec dips),
Dark Purple (6-10 x 1 sec dips)

Question 14

What are indications of a toxic change in neutrophils?

Answer 14

• cytoplasmic basophilia
• cytoplasmic vacuolation
• formation of Dohle bodies

Question 15

Overstaining will make identification of _____
difficult, which is a sign of a regenerative anemia.

Answer 15

polychromasia

Question 16

When compared to canine erythrocytes, feline RBC are ___ and have ___ central pallor.

Answer 16

smaller, less