Hemoglobin Workshop

Question 1

HbC AA composition

Answer 1

• loss of glutamic acid for lysine

- gives it a positive charge now

Question 2

• who is this from?
  prominent HbS
  small amount of HbA/HbA2

Answer 2

• heterozygous for B-thallassemia and B^s mutation

- homozygous for B^s with HbA transfusion

Question 3

• who is this from?
  prominent HbS
  small amount of HbF/HbA2

Answer 3

• patient with sickle cell anemia with slightly elevated HbF

- longer surviving HbF

Question 4

• who is this from?
  prominent HbS and HbA
  small amount HbA2

Answer 4

• patient heterozygous for B^s and B^A

Question 5

• who is this from?
  prominent HbF
  small amount HbA and HbA2

Answer 5

• newborn

Question 6

• who is this from?
  prominent HbA
  small amount of HbA2

Answer 6

• normal adult

Question 7

which form of HbS participates in sickling

Answer 7

• only deoxy form

- T conformation

Question 8

what can alter Hb concentration inside cells?

Answer 8

• dehydration

Question 9

at what pO2 concentration does sickling start to occur

Answer 9

• low pO2 concentration

Question 10

the longer cells are held at a lower pO2

Answer 10

• the greater the amount of polymer formation

Question 11

effect of vasoconstriction or occlusion on sickling

Answer 11

• will increase sickling because cells will spend more time in low pO2 environment

Question 12

slope of curve of depolymerization

Answer 12

• linear
• less step slope
• not cooperative

Question 13

effect of decrease in pH on sickling

Answer 13

• will increase sickling

- acidic environment favors deoxy form

Question 14

effect of increase of temperature on sickling

Answer 14

• will increase sickling