HISTOTECHNOLOGY: EMBEDDING

Question 1

Process by which the impregnated tissue is placed into a precisely arranged position in a mold containing a medium which is then allowed to solidify

Answer 1

EMBEDDING/CASTING/BLOCKING

Question 2

Process by which a tissue is arranged in precise positions in the mold during embedding, on the microtome before cutting, and on the slide before staining

Answer 2

Orientation

Question 3

Temperature of melted paraffin used for embedding

Answer 3

5-10ºC above

Question 4

TYPES OF EMBEDDING MOLDS

Answer 4

• LEUCKHART’S EMBEDDING MOLDS
• COMPOUND EMBEDDING UNIT
• PLASTIC EMBEDDING RING
• METAL BASE MOLD
• DISPOSABLE MOLDS

Question 5

TYPES OF EMBEDDING MOLDS

• 2 L shaped strips of heavy brass or metal
• Adjustable
• Used for large tissues

Answer 5

LEUCKHART’S EMBEDDING MOLDS

Question 6

TYPES OF EMBEDDING MOLDS

• Series of interlocking plates with several compartments
• More than one specimen for batch processing

Answer 6

COMPOUND EMBEDDING UNIT

Question 7

TYPES OF EMBEDDING MOLDS

DISPOSABLE MOLDS

Answer 7

• Peel away
• Plastic ice trays
• Paper boats