HPLC

Question 1

HPLC columns for reversed - phase chromatography separations

Answer 1

HPLC columns are:
-Tubes packed with separation media-optimized to achieve high resolution HPLC separations.

Question 2

The column : column selection types

Answer 2

Reversed phase: retention decreases with increased organic content
Normal phase : retention decreases with increased polar content
Ion exchange : Separation by ionic charge
Gel permeation : Retention changes by size of compound in solution no interaction of material
Ion exclusion : Separation by excluding ions interactions
Affinity : Specific ligands that have binding capacity analyte
HIC : Retention increases with increased organic solvent