HPLC Detector

Question 1

Types of Detectors

Answer 1

UV:
Fluorescence:
Electrochemical:
Refractive Index
Radiochemical:
Mass Spectroscopy

Question 2

UV:

Answer 2

measure the ability of a sample to absorb light; one or several wavelengths

Question 3

Fluorescence:

Answer 3

measure the ability of a compound to absorb then re-emit light at given wavelengths; each compound has a characteristic fluorescence

Question 4

Electrochemical:

Answer 4

measure compounds that undergo oxidation or reduction reactions

Question 5

Refractive Index

Answer 5

measure the ability of sample molecules to bend or refract light; this property for each molecule or compound is called its refractive index.

Question 6

Radiochemical:

Answer 6

involves the use of radiolabelled material, usually tritium (3H) or carbon-14 (14C); operates by detection of fluorescence associated with beta-particle ionisation