HTS (3)

Question 1

HTS +bioassays

Answer 1

• High throughput screening involves using a chemical “library” and testing to activity against your target (100-1000’s of compounds)
• 2 types of lib (combinatorial + natural product)

Question 2

Combinatorial Libraries; pros and cons

Answer 2

• PRO: chemical synthesis = ability to produce lots of compounds at a fast rate
  CON: Structural diversity is limited (all in the same family)
  CON: Difficult separations because of the low structural diversity

Question 3

Natural base products; pros and cons

Answer 3

• Plant/bacterial extracts that contain many diff molecules or compounds
  PRO: Highly diverse set of structures and bioavailable
  CON: Complex mixtures are very hard to isolate (difficult to identify active component)

Question 4

two HTS technologies

Answer 4

SPA - scintillation proximity assay

FLIPR - fluorescent imaging plate reader