Imaging techniques

Question 1

MRI Key Imaging - fMRI

Answer 1

fMRI:

• BOLD (Blood Oxygen Dependent Levels):
• deoxyHb paramagnetic

Question 2

PET Key Imaging

Answer 2

Fluorine 18

• FDG (Flurodeoxyglucose) –> Glucose Usage
• Flurothymidine and Thymidine Kinase: Upreg in proliferating cells for DNA synthesis
• Iodine 125: used with SPECT imaging for apoptosis Signals

Question 3

MRI Key Imaging: dDNP

Answer 3

dDNP: Dissolution Dynamic Nuclear Polarisation

- 13C pyruvate tracking, LDH levels

Question 4

MRI Key Imaging: Angiognesis and Diffusion Weight MRI

Answer 4

Angio-genesis: Gadolinium

Diffusion Weighting MRI: Water and Apparent diffusion Coefficient

Question 5

SPECT imaging: Apoptosis in tumours

Answer 5

Annexin V binds to phosphatidylserine
Binds to Technetium-99m also
Combined with positron emitter such as Iodine-125

Question 6

Fluorescent Imaging - Guiding Surgery

Answer 6

Ovarian Tumours

Folate A receptor: Folate Conjugated to Fluorescein isothiocynate –> (internalised receptor and ligand)