Routinely used for the identification of specific or highly selective cellular epitopes or antigens in frozen or paraffin e embedded tissues
Immunohistochemistry
It can also detect organism cyto logic preparations
Immunihistochemistry
Most commonly used antibody for IHC
IgG
Used of antibodies as histologicals tools for identifying patterns of antigen distribution within a tissue or a sputum sample, fine needle aspirates
Immunohistochemistry
The structural part of the antigen that reacts with antibody
Entetope
Produced by immunizing an anti a, with an immunogen that contains the antigen of interests
Polyclonal antibodies
Most frequently used animal in polyclonal ab
Rabbit
Goat
Pigs
Sheep’s
Horse
Guinea pig
Product of an individual clone of plasma cells
Monoclonal antibodies
Animals used in monoclonal antibodies
Mice
Primary antibody only
Direct method
One step staining method
Direct method
Involves a labeled antibody reacting directly with the antigen in tissue sections
Direct method
Primary and secondary antibodies
Indirect method
Involves an unlabeled
Indirect method
Primary antibody (first layer) which React with tissue antigen and a labeled secondary antibody (second layer) react with primary antibody
Indirect method
