Immunological testing

Question 1

Which immunoglobin is dominant in a secondary response?

Answer 1

IgG

Question 2

What does ELISA stand for?

Answer 2

Enzyme-linked immunosorbent assay

Question 3

What does an ELISA test rely on?

Answer 3

Antibody-antigen interaction

Question 4

What are the benefits of an ELISA test?

Answer 4

• quantifiable
• quick
• reproducible
• inexpensive

Question 5

Describe the steps of an ELISA test

Answer 5

• Coat the plate wells with antigen
• Add test sera samples - will bind in positive animals
• Add enzyme tagged anti-immunoglobulin (secondary antibody) that will bind to positive antigens
• Add substrate and look for a colour change/light signal
• in between each step wash the plate to remove unbound agents

Question 6

What is an end point titration?

Answer 6

A continual dilution of a sample until you can no longer measure antibody
A sample that requires more dilutions had an original higher conc of antibody

Question 7

What determines the size of immune complexes?

Answer 7

• Relative amounts of antigen and antibody
• Number of binding sites on the antigen
• Class of antibody

Question 8

What is the biggest advantage of using immunofluorescence?

Answer 8

More sensitive so can detect lower quantities of what we are looking for

Question 9

What is the most common rapid serological test?

Answer 9

Snap tests

Question 10

Describe how to use a snap test

Answer 10

• Add a sample at one end and look for lines at the other end
• First line shows that the test has worked
• Relies on migration of the serum
• If there are no antibodies then binding wont occur