important

Question 1

what organisms can be modified via “insertion mutagenesis

Answer 1

• plants (plasmid)
• worm (TE)
• fruitfly (TE)
• zebrafish (TE and retrovirus)
• mice (retrovirus in ES cells

Question 2

what organisms can be modified via “homologous recombination”

Answer 2

• yeast (KO, KI)
• fruitfly
• mice (cKO ES cells)

Question 3

what organisms can be modified via CRISPR

Answer 3

• yeast
• plant
• worm
• fruitfly
• zebrafish
• xenopus
• mice

Question 4

what organisms can be modified via “Inducible systems

Answer 4

• yeast (cre-LoxP, Tet on/off)
• plant (cre-LoxP, Tet on/off, FLP-FRT, UAS-GAL4, UAS-EART2-GAL4
• worm (Cre-LoxP, FLP-FRT)
• fruitfly (UAS-Gal4, FLP-FRT)
• zebrafish (UAS-EART2-GAL4)
• xenopus (Tet on/off)
• mice (Cre-LoxP, ERT2-Cre-LoxP, Tet on/off)

Question 5

what organisms can be modified via “RNAi”

Answer 5

• plant
• worm (feeding library)
• fruitfly (UAS-RNAi)
• mice (theoretical)

Question 6

how can yeast be modified

Answer 6

• HR (KO, KI)
• CRISPR
• Inducible systems (Cre-LoxP, Tet on/off)

Question 7

how can plants be modified

Answer 7

• insertion mutagenisis (plasmid)
• CRISPR
• inducible system (5 systems)
• RNAi

Question 8

how can worms be modified

Answer 8

• insertion mutagenesis (TE)
• HR (theoretical)
• CRISPR
• inducible systems (Cre-LoxP, FLP-FRT (theoretical))
• RNAi (feeding library)

Question 9

how can fruitflies be modified

Answer 9

• insertion mutagenesis (TE)
• HR
• CRISPR
• inducible systems (UAS-Gal4,FLP-FRT)
• RNAi (UAS-RNAi

Question 10

how can zebrafish be modified

Answer 10

• insertion mutations (TE, retrovirus)
• CRISPR
• Inducible systems (UAS-ERT2-GAL4)

Question 11

how can xenopus be modified

Answer 11

• insertion mutagenesis (theoretical)
• CRISPR
• inducible systems (Tet on/off)

Question 12

how can mice be modified

Answer 12

• insertion mutagenesis (retrovirus in ES cells)
• HR (cKO ES cells)
• CRISPR
• inducible systems (Cre-LoxP, ERT2-Cre-LoxP, Tet on/off)
• RNAi (theoretical)