Introduction

Question 1

What did CRISPR allow for

Answer 1

made it possible to accuratelyately and efficiently edit the human germ line -

Question 2

Holoprosencephaly (HPE) is a

Answer 2

Disorder of the forebrain development, characterized by failure of the brain to separate into hemispheres during early development

Question 3

HPE has been reported in?

Answer 3

humans
cattle
sheep
pigs
monkeys
dogs
horses

Question 4

HPE factors

Answer 4

• diabetes mellitus in women recognized risk factor
• teratogens- Ethyl alcohol, retinoids acid, cigarette smoking, steroid alkaloids
• sheep- ingestion of steroid alkaloids cyclopamine

Question 5

Mechanism for action of HPE

Answer 5

interference with the response to sonic hedgehog (SHH) transcription factors important in ventral patterning of the neural plate

Question 6

Clinical signs of HPE

Answer 6

• hypernatremia and hyperchloremia
• electroylte abnormalities (increased thirst
• ADHD
• chewing gum fits (oral proprioceptive deficits)
• seizures