1
Q
Characteristics of Assays
A
sensitive
specific
rapid
quantitative
2
Q
As we purify an enzyme
A
- Activity might go up or down
- Specific activity goes up
- Yield should go down
- Purity should go up
3
Q
What is dialysis
A
the removal of excess salt
4
Q
Chromatography
Stationary phase
A
substance that the compo b unds to be separated pass by or interact with
5
Q
Chromatography
Mobile Phase
A
the carrier for the compounds to be separated
6
Q
K= (Ve-Vo)/(Vt-Vo)
A
higher partition coefficience (K) = smaller protein
7
Q
Bigger proteins
A
move faster
move around the beads
8
Q
Medium
A
Some go in the beads, the others go outside the beads
9
Q
Small
A
Move slower
moves through semi porous beads
