What are the 8 steps in viral replication?
- Recognition
- Attachment
- Penetration/entry
- Uncoating
- Macromolecule synthesis
- Assembly
- Budding - if enveloped virus
- Release
What is a VAP?
Viral attachment protein
- Subject to hypermutation (poses Ab immunity challenge)
- Binds cellular receptor –> viral recognition & attachment to host cell
Is recognition/attachment E dependent? Entry?
R&A - passive
Entry - E dep, requires metabolically active cell
What are the 3 patterns of viral entry into host cell?
- Envelope fuses @ plasma membrane - immediate release of capsid+genetic material
- Enveloped virus taken up as endosome - fusion in endosome to release capsid+genetic
- Naked virus in endosome & released by decreased pH
What is the order of macromolecule synthesis?
- Early mRNA + regulatory proteins
- Genome replication - cytoplasm or nucleus
- Late mRNA + structural proteins
- Modify proteins made
What is the eclipse period of viral growth?
Once virus has entered host cell but disassembled - no virions
What is the latent period of viral growth?
Between viral infection –> detection as extracellular virus (aka all intracell time)
What type of viruses have a detectable intracellular period of viral growth?
Viruses released by cell lysis
What is burst size?
Ratio
Infected virus particles released : cell number
What is the cytopathic effect?
Changes to host cell due to viral infection
Ex:
- Herpes - syncytia
- Flu - RBCs together
What is the genome of adenovirus? Encapsulated?
dsDNA
Naked icosahedral
What diseases does adenovirus case?
Pink eye
RTIs, diarrhea, hemorrhagic cystitis
What is the immune response to adenovirus?
Abs
What is the genome of polyviruses?
dsDNA
Replicates @ cytoplasm
What diseases are caused by polyviruses?
Small pox
Vaccine eradicated this disease
