LAB 1 MICROSCOPE

Question 1

What are microscopes used for?

Answer 1

They are used to see small objects

Question 2

1. What is a compoud microscope?
2. What does ‘compound’ in reference to a microscope means?

Answer 2

1. A microscope that uses 2 or more lens to enlarge an object
2. Two (2) or more lens

Question 3

What is a light microscope? (compound light microscope)

Answer 3

uses a lamp/light to illuminate the specimen

Question 4

List 3 ways to care (safety) for the microscope?

Answer 4

1. carry by holding the base and arm (microscopes are top heavy)
2. only use lens tissue and cleaner
3. Dont let the cord drop to the floor
   Additional - reset scanning objective in place (5x), lower stage, wrap cord

Question 5

Name the different types of microscopes and their uses?

Answer 5

-Compound microscope
most common

-Phase-contrast microscope
used for viewing unstained or transparent specimens

-Fluorescent microscope
Used for viewing specimens stained with a special dye

-Electron microscope
found in hospital, need special training

Question 6

Name the 4 objective lenses, their power and colors?

Answer 6

1. Scanning objective/5x/Red

2.Low power objective/10x/Yellow

3.High power objective/40x/Blue

4.Oil immersion objective/100x/White

Question 7

Parts and Functions of the microscope

Answer 7

Next slide»»»»>

Question 8

what is the function of the arm?

Answer 8

Supports microscope, hold when carrying

Question 9

What is the function of the ‘base’?

Answer 9

sits on table, support when carrying

Question 10

what is the fuction of the ‘stage’?

Answer 10

surface that hold slides and specimen with slide clip

Question 11

what is the function of the ‘upper mechanical stage knob?

Answer 11

moves the slide forward and backward

Question 12

what is the function of the ‘lower mechanical stage knob’?

Answer 12

moves slide left and right

Question 13

what is the function of the ‘coarse adjustment knob’?

Answer 13

moves stage up and down in large increments, used for general focusing.

Question 14

what is the function of the ‘fine adjustment knob’?

Answer 14

moves stage up and down in small increments, used for sharp/detailed focusing

Question 15

what is the function of the ‘condenser’?

Answer 15

concentrates light to the microscope slide

Question 16

what is the function of the ‘Iris Diaphragm lever’?

Answer 16

increase and decrease light to the specimen

Question 17

what is the function of the ‘lamp’?

Answer 17

light source

Question 18

what is the function of the “on and off switch”?

Answer 18

turns lamp on and off

Question 19

what is the function of the ‘brightness control knob’?

Answer 19

increase and decrease light from the lamp

Question 20

what is the function of the ‘Revolving Nose Piece’?

Answer 20

support objective lenses, allow changing of lenses

Question 21

what are the objective lenses?

Answer 21

4 lenses, used to magnify objects

Question 22

1.what is the function of the ‘Eyepiece’ (ocular)?

2.what is the power of the ocular lens?

Answer 22

1.contains the ocular lens
(closes to the eye-one eyepiece may have a pointer)

2.has a power of 10x - shows the item 10 times bigger

Question 23

what are 3 rules to follow when using the microscope?

Answer 23

1. never use the immersion objective without immersion oil
2. always begin focusing with the scanning objetive and stage all the way down
   3.never adjust the coarse adjustment knob while using low or higher objective lens

Question 24

Microscope Basics - FACTS

Answer 24

the image is turned upside down and reversed due to the objective lens, a mirror and the ocular lens.

Question 25

MICROSCOPE TERMINOLOGY

Answer 25

NEXT CARD>>>>>>>>

Question 26

What is "Total Magnification"? and how does it works?

Answer 26

the power of the objective lens multiplied by the power of the ocular lens Total Mag = Ocular power X Objective power Eg: Using high power: 10 X 40 = 400x (10 ocular lens 'multiplied by' 40 high power lens = 400 times total magification)

Question 27

what is 'field of vision'?

Answer 27

Amount of specimen and surrounding area that can be seen. (it changes with each objective)

Question 28

TRUE OR FALSE? 1.The field of vision DECREASES with inreasing magnification? 2. Amount of details INCREASES with increasing magnification?

Answer 28

1.TRUE 2. TRUE

Question 29

what is 'Parfocal lenses'?

Answer 29

Allow objective lenses to stay in focus NOTE - if switched from scanning-to-low power-to-high power the object stays in focus 5x>>10x>>40x (fine adjustment should only be needed)

Question 30

TRUE OR FALSE? The coarse adjustment knob is not needed when using objectives greater than the scanning objective

Answer 30

TRUE

Question 31

whis is "working distance'?

Answer 31

distance between the stage/specimen and the objective lens

Question 32

what is 'Depth of view'?

Answer 32

ability to distinguish closer and deeper structures

Question 33

what is a 'Binicular'?

Answer 33

instrument that allows the use of both eyes

Question 34

STEPS IN FOCUSING USING THE SCANNING OBJECTIVE

Answer 34

1. stage should be all the way down 2. scanning objetive should be in place 3. Place the slide on stage 4. Center and adjust light 5. Raise stage all the way up 6. Focus downward using the coarse adjustment knob 7. Center and adjust light 8. Fine focus using the fine adjustent knob 9. center and adjust light (if necessary)

Question 35

STEPS TO REMOVE SLIDE AND FINISH

Answer 35

1. Rotate scanning objective in place 2. Lower stage using coarse adjustment knob 3. Remove slide 4. Turn off lamp 5. Unplug cord and wrap around the microscope 6. Put back in place on shelve 3x3x3x4