1
Q
what was known as the mobile phase?
A
the solvent
2
Q
what was the solvent?
A
70/30 hexane acetone
3
Q
was the solvent polar or nonpolar?
A
nonpolar
4
Q
what was known as the stationary phase?
A
silica plate
5
Q
is the silica plate polar or nonpolar?
A
polar
6
Q
what if the formula for Rf?
A
distance traveled by compound/ distance traveled by solvent
7
Q
the relative _____________ of compounds is based on ___________ ____________.
A
polarity
distance traveled
8
Q
less polar compounds travel __________
why?
A
further
- due to high affinity for less polar mobile phase
9
Q
what is the expected Rf value of a less polar compound?
A
high Rf value
10
Q
more polar compounds travel ___________
why?
A
less further
- due to high affinity for stationary phase
11
Q
what is the expected Rf value of a more polar compound?
A
a smaller Rf value
Biochemistry II Lab
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