Lab Flashcards

(48 cards)

1
Q

Mono chloramine symbol

A

NH2Cl

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2
Q

What’s the strength of hypochlorite ion OCl- compared to hypochlorous acid?

A

1/100

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3
Q

What’s the strength of monochloramine compared to hypochlorous acid?

A

1/150

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4
Q

What defines precision?

A

When I repeat it do I get the same thing? Replicate samples check for Precision, the consistency of results

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5
Q

2.20 for pocket 2 and +++++ for the 900

A

Out of range

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6
Q

What is quality assurance?

A

A program that defines what actions are required to assure the analytical results are acceptable

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7
Q

What is quality control?

A

Performing specific actions identified in the QA program. (Procedures)

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8
Q

When do you calibrate a pH meter, or any other analysis?

A

When you get a new batch of samples

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9
Q

What’s the equation for % recovery?

A

Sample Result / True Value. * 100

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10
Q

What’s the least accurate glass?

A

Beaker

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11
Q

What’s the name and symbol for trichloramine?

A

Nitrogen Trichloride NCL3

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12
Q

What’s the symbol for dichloramine?

A

NHCl2

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13
Q

What is the titrant when titrating for alkalinity?

A

Sulfuric acid

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14
Q

What is the relationship between the method detection limit and the instrument detection limit?

A

Method is always higher than the instrument

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15
Q

What is the Instrumental ldl when dpd testing?

A

0.1 mg/L

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16
Q

Precision

A

How close together the results of your repeat samples are. Consistency of results

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17
Q

What is the purpose of Known QC solutions?

A

Determines how close to the true value am I? Accuracy

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18
Q

Accuracy

A

How close to the TV am I? Known check samples check for accuracy, or how close the result is to the actual value

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19
Q

What is the Purpose of controls?

A

So you know the analysis parameters in order to determine if the analysis is in or out of control

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20
Q

Things that impact getting a representative sample

A

Store temp incorrectly, sample site isn’t a good place, poor sampling training, exceeding hold time,

21
Q

The other way to test alkalinity?

A

pH instead of color change

22
Q

MPN?

A

A statistical method of Estimating the concentration of bacteria in a liquid sample

23
Q

95% Confidence Limit?

A

The True Value will be within the range 95% of the time.

24
Q

Autoclave temp?

25
What 3 factors do incubators control?
Temperature, humidity, and light
26
Water hardness measures _________, primarily calcium and magnesium.
total dissolved cations in the water
27
What is the preferred number of colonizes on a HPC
30 to 300 CFUs
28
What temp is the agar melted at for a HPC?
45⁰
29
What temperature are TS crucibles dried at?
104⁰
30
What is a heterotrophic organism?
One that uses carbon as a food source.
31
What selective media is used in the Presumptive test?
Laurel Tryptose Broth (LTB)
32
What selective media is used in the Confirmed test?
Brilliant Green Lactose Bile Broth
33
What Selective Media is used in the Completed test?
Plates of Eosin Methylene Blue, then LTB
34
How are test tubes set up initially for the Presumptive test?
Use LTB. Put 10 ml in 5 test tubes, put 1 ml in 5 tubes, and 0.1 ml in 5 test tubes. The 5-5-5 method
35
What information is on the Chain of Custody?
Sampler and Lab: Name, date, time, signature Sample Location Recipients Location Required Analysis and Results
36
What is the Titrant for Total Hardness?
Ethylenediamenetetraacetic acid
37
What pH must a hardness titration be buffered to?
10 pH
38
What is the indicator for a Total Hardness Titration?
ManVer 2 hardness indicator
39
What temperature are coliform incubators set at?
35⁰ C +/- 0.5⁰ C
40
What is a laboratory fortified, or spiked sample, and what does it test for?
A sample with a known quantity of analyte, to test for matrix effects, or if components of a sample matrix interfere with the measurement of the analyze.
41
Solution Concentration =
Instrument result x total volume / sample volume
42
What QC checks for accuracy?
Known check samples
43
What QC checks for precision?
Replicate analysis, the same sample analyzed multiple times
44
What's a replicate sample?
Multiple samples collected at the same sample time
45
What do replicate samples determine?
The precision of all aspects of the analysis
46
Define true value
The known concentration of the analyte of interest in a check sample
47
What's the difference between partial and total alkalinity?
Partial is when all the hydroxide and carbonate are combined with acid and total is when the bicarbonate is all combined with the acid
48
What are the 2 indicators when titrating alkalinity?
Phenolphthalein and Bromcresol Green-Methyl Red