Lab 6 Pulmonary Function

Question 1

What is the name of the device used that’ll measure breathing

Answer 1

A-FH-300L Spirometer flowhead and plastic tubes

Question 2

How to setup the spirometer

Answer 2

1. Push the 2 air flow tubes onto the 2 outlets on the A-FH-300L Spirometer
2. Connect other ends of the air flow tubes into Channel A1 of IXTA. Connect red port to red connector on tubing

Question 3

What must be done before starting ex 1 what should u do

Answer 3

1. Subject must be healthy and no history of respiratory or CV problems
2. Outlets connected to the airflow tubing should be pointed up to avoid condensation developing
3. Use nose-clip to prevent air from entering/leaving nose as subject breathes. Air that passes thru nose cause errors in lung volume values
4. Calibrate spirometer on “Lung Volumes” channel
5. Allow IXTA warm up 10 mins before recording 1st time

Question 4

When spirometry data recorded, inhalation and exhalation are displayed how

Answer 4

1. inhalation displayed as upward deflection

2. exhalation displayed as downward deflection

Question 5

How to determine if subject breathing in correct end of flow tube

Answer 5

1. Click on preview mode and record
2. Have subject inhale
3. If flowhead oriented properly, traces on Air Flow and Lung Volume channels go up during inhalation
4. If traces on channels go down, breathe in other end of flowhead or reverse airflow tubes position at outlets of flow head

Question 6

What is exercise 1

Answer 6

Breathing while resting

Question 7

What is the aim of ex 1 breathing while resting

Answer 7

To measure BREATHING PARAMETERS in healthy, RESTING subject

Question 8

Time for ex 1 breathing while resting

Answer 8

20 mins

Question 9

Procedure for ex 1 breathing while resting

Answer 9

1. Subject SIT quietly, become accustomed to breathing thru spinometer flow head, breathe normally. Hold flow head so outlets pointed up. Remove flow head from mouth and hold at mouth level in position that prevents breath from moving thru flow head.
2. Click record. Wait 10 secs for Lung Volumes channel to zero, then subject breathe in flow head with mouth.
3. Record 5-6 breaths, takes ~45 secs.
4. Subject inhale as deeply as possible. When reach max inhalation volume, exhale quickly and completely.
5. Then breathe normally for 5-6 breaths

Question 10

Set up for ex 2?

Answer 10

1. Connect ends of air flow tubes into Channel A1 like in exp 1
2. Connect DIN8 connector of PT-104 plethysmograph into Channel 5
3. Place plethysmograph on volar surface of distal segment of middle finger/thumb

Question 11

What must be done before starting ex 2 what should u do

Answer 11

1. Subject must be healthy and no history of respiratory or CV problems
2. Outlets connected to the airflow tubing should be pointed up to avoid condensation developing
3. Use nose-clip to prevent air from entering/leaving nose as subject breathes. Air that passes thru nose cause errors in lung volume values
4. Calibrate spirometer on “Lung Volumes” channel
5. Allow IXTA warm up 10 mins before recording 1st time

Question 12

What is ex 2 called

Answer 12

HR while breathing at rest

Question 13

Aim of ex 2 HR while breathing at rest

Answer 13

To determine EFFECT of BREATHING WHILE AT REST on subject’s HR and change in HR during RESPIRATORY SINUS ARRHYTHMIA (RSA)

Question 14

Time for ex 2 HR while breathing at rest

Answer 14

30 mins

Question 15

Procedure for ex 2 HR while breathing at rest

Answer 15

1. Subject SIT quietly, become accustomed to breathing thru spinometer flow head, breathe normally. Hold flow head so outlets pointed up. Remove flow head from mouth and hold at mouth level in position that prevents breath from moving thru flow head.
2. Click record. Wait 10 secs for Lung Volumes channel to zero, then subject breathe in flow head with mouth.
3. Record 5 breaths, takes ~45 secs.
4. Subject inhale as deeply as possible. When reach max inhalation volume, exhale quickly and completely.
5. Then breathe normally for 5-6 breaths

Question 16

Why may there by sections of data on the Lung Volumes channel where baseline is sloped

Answer 16

Sloping occurs when temp inside spirometer inc while subject breathes thru unit

Question 17

What is the purpose of using the SpiroLab II Spirometry System when we’ve just used IWRX

Answer 17

Measure many of the same variables measured previously with iWorx flowhead. Allows u to compare and contrast 2 diff sets of equipment, methods, and results

Question 18

What 6 things does the SpiroLab II Spirometry measyre

Answer 18

1. Vital capacity (VC)
2. Forced vital capacity (FVC)
3. Forced expiratory volume in 1 sec (FEV1)
4. FEV1.0/FVC percent
5. Maximal midexpiratory flow rate (MMFR), called FEF25-75% by the computer. Stands for forced expiratory flow rate from 25 to 75% of forced vital capacity curve.
6. Peak expiratory flow rate (PEF): maximal flow rate obtained during FEFV1 maneuver

Question 19

Fxn and accuracy of SpiroLabII Spirometry System

Answer 19

• A computerized system for performing pulmonary fxn measurements.
• Accuracy for volume measurements: +/- 3%
• Accuracy for flow rate measurements: +/- 5%
• Small error in calculation of BTPS cause barometric pressure at SFU varies btwn 715-745 mmHg, and machine assumes barometric pressure of 760 mmHg

Question 20

What is ex 3 called

Answer 20

Performing the pulmonary fxn measurements

Question 21

Procedure for Ex 3

Answer 21

1. Subject perform forced vital capacity test (FVC) three times.
2. Only expire into automated spirometer, do not inspire

Question 22

What is ex 4 called

Answer 22

Calibrate the iWire GA Gas Analyzer

Question 23

Procedure for ex 4 calibrate the iWire GA Gas Analyzer

Answer 23

• Warm up gas analyzer 15 mins before using. Make sure calibration gas tank near gas analyzer

1. Connect gas sample tubing to connector on the output of the regulator
2. Click on Calibrate Gas Analyzer
3. Click on Perform Quick Software Gas Calibration
4. Follow directions. Room air will be sampled for 10 secs. Calibration gas will be sampled for 15 secs.
5. If necessary, move cursors in correct position

Question 24

What is Ex 5 called

Answer 24

Breath-Holding Exp

Question 25

What is the procedure for ex 5 Breathing-holding Exp

Answer 25

1. Calibrate iWire-GA Gas Analyzer 2. Treatment #1: at end of normal breath, exhale continuously to collect alveolar gas sample. Record FAO2, FACO2 REST 2 MINS TO ALLOW ALVEOLAR GASES TO RETURN TO NORMAL LVLS 3. Treatment #2: hold breath for as long as possible from the end of normal inspiration. collect alveolar gas sample. Record FAO2, FACO2, and breath hold-time. REST 2 MINS TO ALLOW ALVEOLAR GASES TO RETURN TO NORMAL LVLS 4. Treatment #3: take deep inspiration and hold for as long as possible. collect alveolar gas sample. Record FAO2, FACO2, and breath hold-time REST 2 MINS TO ALLOW ALVEOLAR GASES TO RETURN TO NORMAL LVLS 5. Treatment #4: take deep inspiration of oxygen from 10L balloon and hold breath for as long as possible. collect alveolar gas sample. Record FAO2, FACO2, and breath-hold time. REST 2 MINS TO ALLOW ALVEOLAR GASES TO RETURN TO NORMAL LVLS 6. Treatment #5: subject hyperventilate by prolonged rapid and deep breathing for 1 min, then hold breath as long as possible. Monitor alveolar O2 and CO2 conc both at end of hyperventilation, and after breath holding. Record FAO2, FACO2, and breath hold time

Question 26

During hyperventilation why should subject sit in chair

Answer 26

In case syncope (fainting) occurs