LAB 7 - Bacteria

Question 1

What are prokaryotes?

Answer 1

• unicellular microrganisms

- lack nucleus and membrane bound organelles

Question 2

What are the two types of bacteria?

Answer 2

Gram+

Gram-

Question 3

What is the size of bacteria?

Answer 3

.5-5 micrometers

Question 4

Bacteria is a _______

Answer 4

domain

Question 5

What are plasmids?

Answer 5

-small, circular, double-stranded DNA molecules

Question 6

What are the 3 antibiotics tested in lab?

Answer 6

1. Penicillins
2. Tetracyclines
3. Aminoglycosides (streptomycin)

Question 7

What are 4 steps of antibiotic resistance?

Answer 7

1. lots of germs, few are drug resistant
2. antibiotics kill good and bad bacteria
3. drug resistant bacteria now allowed to grow + take over
4. some bacteria give their drug-resistance to other bacteria

Question 8

What were the 2 bacteria tested in lab?

Answer 8

1. Escherichia coli (E. coli)

2. Staphylococcus aureus (S. aureus)

Question 9

What was the agar gel used?

Answer 9

TSA

tryptic soy agar

Question 10

What is a zone of inhibition?

Answer 10

• area around antibiotic disk

- where bacterial growth has been inhibited

Question 11

What are the ranges for the zone of inhibition?

Answer 11

resistant <15mm
intermediate 16-20mm
susceptible >21mm