Lab: Bacteria

Question 0

Gram stain of E. coli

Answer 0

Gram (-)

Question 1

What is the morphology of E. coli?

Answer 1

Bacilli

Question 2

Colony appearance of E. coli

Answer 2

Beige colonies

Question 3

Details of E. coli

Answer 3

• most commonly worked with bacteria
• most strains harmless, typically hear about O157:H7 that causes food poisoning
• ubiquitous
• part of Enterobacteriaceae family
• molecular “workhorse”

Question 4

What’s the morphology of S. aureus?

Answer 4

Cocci

Question 5

Gram stain of S. aureus

Answer 5

Gram +

Question 6

Colony appearance of S. aureus

Answer 6

Golden yellow

Question 7

Details of S. aureus

Answer 7

• golden grape bunches
• normal part of skin flora but typically hear about MRSA
• staph typically blamed for Strep infections

Question 8

Morphology of S. mutans

Answer 8

Cocci

Question 9

Gram stain of S. mutans

Answer 9

Gram +

Question 10

Colony appearance of S. mutans

Answer 10

Opaque colonies

Question 11

Details of S. mutans

Answer 11

• normal part of oral flora
• responsible for carb metabolism thus creating an acidic environment that contributes to dental caries
• forms dental biofilms

Question 12

Morphology of P. hauseri

Answer 12

Bacilli

Question 13

Gram stain of P. hauseri

Answer 13

Gram -

Question 14

Colony appearance of P. hauseri

Answer 14

Opaque colonies

Question 15

Details of P. hauseri

Answer 15

• highly motile
• found in intestines of humans and animals, soil and water
• known to cause UTIs

Question 16

Morphology of B. cereus

Answer 16

Bacilli

Question 17

Gram stain of B. cereus

Answer 17

Gram +

Question 18

Colony appearance of B. cereus

Answer 18

Fluffy white colonies

Question 19

Details of B. cereus

Answer 19

• often used as probiotic for animals
• harmless except for strains that cause fried rice syndrome
• can produce endospores

Question 20

Morphology of B. megaterium

Answer 20

Bacilli

Question 21

Gram stain of B. megaterium

Answer 21

Gram +

Question 22

Colony appearance of B. megaterium

Answer 22

White with ruffled edges

Question 23

Details of B. megaterium

Answer 23

• one of largest bacteria in terms of size
• naturally produces penicillin amidase
• can produce spores

Question 24

Morphology of B. subtilis

Answer 24

Bacilli

Question 25

Gram stain of B. subtilis

Answer 25

Gram +

Question 26

Colony appearance of B. subtilis

Answer 26

Dull, tan, raised, wrinkled edges

Question 27

Details of B. subtilis

Answer 27

- found in soil and vegetation - model organism for the study of endospore formation - can be used as fungicide - naturally produces new phospholipid antibiotic - degrades TNT

Question 28

Morphology of K. rosea

Answer 28

Cocci

Question 29

Gram stain of K. rosea

Answer 29

Gram +

Question 30

Colony appearance of K. rosea

Answer 30

Pink colonies

Question 31

Details of K. rosea

Answer 31

- aka Micrococcus roseus - found in freshwater, saltwater and soil - chromogenesis at 25 to form pink colonies - fastidious

Question 32

Morphology of M. luteus

Answer 32

Cocci

Question 33

Gram stain of M. luteus

Answer 33

Gram +

Question 34

Colony appearance of M. luteus

Answer 34

Yellow colonies

Question 35

Details of M. luteus

Answer 35

- aka Kocuria rhizophila - typical soil bacterium - threat to immunocompromised individuals - chromogenesis at 25 to form yellow colonies

Question 36

Morphology of S. marcescens

Answer 36

Bacilli

Question 37

Gram stain of S. marcescens

Answer 37

Gram -

Question 38

Colony appearance of S. marcescens

Answer 38

Red colonies

Question 39

Details of S. marcescens

Answer 39

- produces prodigiosin at 25 giving red appearance - very ubiquitous - can be pathogen (UTI and catheter) - US and UK tested effects by spraying - church wafers

Question 40

Morphology of P. aeruginosa

Answer 40

Bacilli

Question 41

Gram stain of P. aeruginosa

Answer 41

Gram -

Question 42

Colony appearance of P. aeruginosa

Answer 42

Opaque green/blue colonies

Question 43

Details of P. aeruginosa

Answer 43

- ubiquitous human and plant pathogen - produces pyocyanin (blue-green) at 37 - loves to be in biofilms - CF and burn patients

Question 44

Morphology of E. faecalis

Answer 44

Cocci

Question 45

Gram stain of E. faecalis

Answer 45

Gram +

Question 46

Colony appearance of of E. faecalis

Answer 46

Opaque colonies

Question 47

Details of E. faecalis

Answer 47

- typical GI bacterium - found in cosmetics and hygienic chemicals - ability to spoil sap and syrup - part of Enterobacteriaceae family

Question 48

Morphology of P. phosphoreum

Answer 48

Bacilli

Question 49

Gram stain of P. phosphoreum

Answer 49

Gram -

Question 50

Colony appearance of P. phosphoreum

Answer 50

Blue-green glowing colonies

Question 51

Details of P. phosphoreum

Answer 51

- highly motile - symbiotic, often found in deep sea fish - used to assess biological toxicity within a system