Lab Compre (RVSP)

Question 0

Veins for venipuncture

Answer 0

Cephalic vein
Basilic vein
Median cubital vein

Question 1

Ideal puncture depth

Answer 1

2-3 mm

Question 2

Inches above the site the tourniquet is applied

Answer 2

3-4 in

Question 3

Angle in venipuncture

Answer 3

15°

Question 4

Ideal venipuncture depth

Answer 4

0.5 - 1.0 cm

Question 5

Order of draw accdg to color

Answer 5

Yellow
Light blue
Red
Green
Lavender
Gray

Question 6

Oder of draw accdg to function

Answer 6

Blood culture
Coagulation
Serology/BB
Plasma chemistry
Blood cell counting
Glucose

Question 7

Order of draw accdg to additives

Answer 7

ACD & SPS
Sodium citrate
None
Sodium/lithium heparin
K2EDTA
Sodium fluoride-potassium oxalate

Question 8

Additive; inhibits thrombin formation to prevent clotting

Answer 8

Sodium heparin/Lithium heparin

Question 9

Needle angle for arterial puncture

Answer 9

35-40°

Question 10

Most popular method for smear preparatiom

Answer 10

Two-slide/Wedge method

Question 11

Angle of spreader slide in smear prep

Answer 11

30-45°

Question 12

4 criterion for a good smear

Answer 12

• even transition from thick to thin area
• must cover 2/3 or 3/4 of the slide
• smooth and even surface, free from ridges, waves, or holes
• feathery edge

Question 13

Other mtds for smear prep

Answer 13

• Two-cover slip/Ehrlich’s Two-cover glass method
• Spinner’s method
• Automated smear preparation

Question 14

Instrument used for Spinner’s mtd of smar prep

Answer 14

Hemaspinner

Question 15

Method for staining blood smear

Answer 15

Staining jar/Dip method

Question 16

Stains in the Staining jar/dip method

Answer 16

Methanol
Eosin
Methylene blue
Buffer solution/aged distilled water

Question 17

4 mtds for differential counting

Answer 17

Four-field meander
Two-field
Exaggerated battlement
Strip differential

Question 18

6 stations in the Hemastainer Automatic Slide Stainer

Answer 18

• Methanol
• Wright’s or Wright-Giemsa stain
• Stain-Buffer mixture (Wright’s or Wright-Giemsa + Phosphate buffer)
• Deionized water
• Phosphate buffer
• Warm air

Question 19

Mtd of staining; blood smear is placed on a rack positioned in a dish

Answer 19

Staining Dish Mtd

Question 20

Contained in the Stain-Pak

Answer 20

Stain
Buffer
Rinse solution

Question 21

Staining mtd; uses Stain-Pak

Answer 21

• Hema-Tek 1000 Slide Stainer

- Hema-Tek 2000 Slide Stainer

Question 22

Stain for diff ct; contains methylene blue or its oxidative product like Azure B; also contains Eosin B or Eosin Y

Answer 22

Romanowsky Stains

Question 23

Most satisfactory type of Romanowsky stain for general routine hematologic studies

Answer 23

Wright’s stain

Question 24

Composition of Wright's stain

Answer 24

Oxidized methylene blue | Eosin azures

Question 25

Stain for demonstration of inclusion bodies and intracellular parasites aside from staining WBCs

Answer 25

Giemsa stain

Question 26

Composition of Giemsa stain

Answer 26

Eosin Y with Azure blue | Methylene blue in methanol with glycerin

Question 27

Stain for diff ct; similar to Wright's stain except for the mtd used to oxidize methylene blue

Answer 27

Leishman, Jenner, and May-Grunwald

Question 28

Stains that consist of Romanowsky stain and another dye

Answer 28

Panoptic stains

Question 29

Types of panoptic stains

Answer 29

Wright's-Giemsa Jenner-Giemsa May-Grunwald-Giemsa

Question 30

Hb is measures as

Answer 30

Oxyhemoglobin

Question 31

Indirect mtds for measuring Hb

Answer 31

- Acid Hematin mtd | - Cyanmethemoglobin (MHbCN) mtd or Hemoglobin Cyanide (HiCN) mtd

Question 32

Reagent in Acid Hematin mtd for Hb

Answer 32

0.1 N HCl

Question 33

Color of soln in Acid Hematin Mtd

Answer 33

Yellowish Brown

Question 34

Reagent used in the Cyanmethemoglobin mtd or Hemoglobin cyanide mtd for Hb

Answer 34

Drabkin's reagent

Question 35

Composition of Drabkin's reagent

Answer 35

- potassium ferricyanide - potassium cyanide - sodium bicarbonate

Question 36

Best manual metd for Hb determination

Answer 36

Cyanmethemoglobin or Hemoglobin cyanide mtd

Question 37

Form of Hb not converted to cyanmethemoglobin by Drabkin's reagent

Answer 37

Sulfhemoglobin

Question 38

Wavelength in cyanmethemoglobin mtd

Answer 38

540 nm

Question 39

Direct matching mtds for Hb

Answer 39

Tallquist or Scale Dares Hemoglobinometer Alkaline Hematin

Question 40

Direct mtds for Hb

Answer 40

- Oxyhemoglobin (Sodium Carbonate and Photoelectric Oxyhemoglobin) - Carboxyhemoglobin